Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Candida tropicalis critically affects Candida albicans virulence by limiting its capacity to adhere to the host intestinal surface leading to decreased susceptibility to colitis in mice

Kyle Roberts
ORCID logo ,
Abdullah Osme
,
Carlo De Salvo
,
Eleonora Zoli
,
Janet Herrada
,
Thomas McCormick
,
Mahmoud Ghannoum
,
Fabio Cominelli
,
Luca Di Martino
*
Version 1 : Received: 28 February 2024 / Approved: 29 February 2024 / Online: 29 February 2024 (10:53:24 CET)

How to cite: Roberts, K.; Osme, A.; De Salvo, C.; Zoli, E.; Herrada, J.; McCormick, T.; Ghannoum, M.; Cominelli, F.; Di Martino, L. Candida tropicalis critically affects Candida albicans virulence by limiting its capacity to adhere to the host intestinal surface leading to decreased susceptibility to colitis in mice. Preprints 2024, 2024021714. https://doi.org/10.20944/preprints202402.1714.v1 Roberts, K.; Osme, A.; De Salvo, C.; Zoli, E.; Herrada, J.; McCormick, T.; Ghannoum, M.; Cominelli, F.; Di Martino, L. Candida tropicalis critically affects Candida albicans virulence by limiting its capacity to adhere to the host intestinal surface leading to decreased susceptibility to colitis in mice. Preprints 2024, 2024021714. https://doi.org/10.20944/preprints202402.1714.v1

Abstract

Candida (C.) infections represent a serious health risk for people affected by inflammatory bowel disease. An important fungal virulence factor is the capacity to form biofilms on the colonized surface of the host. This research study aimed to determine the effect of C. tropicalis and C. albicans co-infection on dextran sodium sulfate (DSS)-induced colitis in mice. Colitis severity was evaluated using histology and colonoscopy. Mice were mono-inoculated with C. albicans, C. tropicalis or co-challenged with both species. Mice were administered 3% DSS to induce acute colitis. Biofilm activity was assessed using (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl] 2H-tetrazoliumhydroxide (XTT) assay. Abundance of C. albicans in the colon tissues was assessed by immunohistochemistry. Co-challenged mice showed decreased colitis severity compared to mono-inoculated mice. The XTT assay demonstrated a marked decrease of C. albicans biofilm production in a C. albicans culture incubated with C. tropicalis supernatant. Immunohistochemical staining showed that C. albicans was more abundant in the mucosa of C. albicans mono-inoculated mice compared to the co-inoculated group. These data indicate an antagonistic microbial interaction between the two Candida species, where C. tropicalis may produce molecules capable of limiting the ability of C. albicans to adhere to the host intestinal surface leading to a reduction in biofilm formation.

Keywords

C. albicans; colitis; C. tropicalis; mycobiome; biofilm

Subject

Biology and Life Sciences, Immunology and Microbiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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