Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Taxonomic Identification and Antagonistic Mechanism of Streptomyces Luomodiensis Sp. Nov. Against Phytopathogenic Fungi

Dengfeng Qi
* ORCID logo ,
Qiao Liu
,
Liangping Zou
,
Miaoyi Zhang
,
Kai Li
,
Yankun Zhao
,
Yufeng Chen
,
Junting Feng
,
Dengbo Zhou
,
Yongzan Wei
,
Wei Wang
,
Lu Zhang
,
Jianghui Xie
Version 1 : Received: 28 February 2024 / Approved: 28 February 2024 / Online: 29 February 2024 (17:02:36 CET)

How to cite: Qi, D.; Liu, Q.; Zou, L.; Zhang, M.; Li, K.; Zhao, Y.; Chen, Y.; Feng, J.; Zhou, D.; Wei, Y.; Wang, W.; Zhang, L.; Xie, J. Taxonomic Identification and Antagonistic Mechanism of Streptomyces Luomodiensis Sp. Nov. Against Phytopathogenic Fungi. Preprints 2024, 2024021660. https://doi.org/10.20944/preprints202402.1660.v1 Qi, D.; Liu, Q.; Zou, L.; Zhang, M.; Li, K.; Zhao, Y.; Chen, Y.; Feng, J.; Zhou, D.; Wei, Y.; Wang, W.; Zhang, L.; Xie, J. Taxonomic Identification and Antagonistic Mechanism of Streptomyces Luomodiensis Sp. Nov. Against Phytopathogenic Fungi. Preprints 2024, 2024021660. https://doi.org/10.20944/preprints202402.1660.v1

Abstract

Banana wilt caused by Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4) is a devastating fungal disease. Biocontrol strategies owned immense potential in inhibiting the spread of Foc TR4. Here, thirty actinobacteria were isolated from soils and screened for their antagonistic activity against Foc TR4. Strain SCA4-21T exhibited broad-spectrum antifungal activity against nine phytopathogenic fungi including Foc TR4. The strain was identified as the genus Streptomyces according to phenotypic characteristics and 16S rRNA gene phylogenetic analysis. Multi-locus sequence analysis based on five house-keeping gene alleles (atpD, gyrB, recA, rpoB, and trpB) revealed that strain SCA4-21T clustered into S. hygroscopicus subsp. hygroscopicus NBRC 13472T with 100% of bootstrap value. Average nucleotide identity and dDDH were 98.49% and 44.30% above the threshold of novel species. Hence, strain SCA 4-21T represented a novel species within the genus Streptomyces, named as Streptomyces luomodiensis sp. nov.. The type strain is SCA4-21T (=GDMCC4.340T= JCM36555T). By the CAZymes analysis, 348 carbohydrate-active enzymes were detected, including 15 chitinases and 8 β-1,3-glucanases. The fermentation broth of strain SCA4-21T showed high activities of chitinase and β-1,3-glucanase, contributing to the antifungal activity. Our results showed an innovative potential biocontrol agent for managing plant fungal diseases, specifically banana fusarium wilt.

Keywords

Broad-spectrum antifungal activity; Taxonomic identification; CAZymes analysis; Streptomyces luomodiensis sp. nov. Fungal cell wall degradation

Subject

Biology and Life Sciences, Life Sciences

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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