Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Fermentative utilization of glycerol by E. coli and its implications in biodiesel industry

Version 1 : Received: 28 February 2024 / Approved: 28 February 2024 / Online: 28 February 2024 (17:02:18 CET)

How to cite: Carvajal, O.C.; Montes de Oca, J.M.G.; Moreno, D.C. Fermentative utilization of glycerol by E. coli and its implications in biodiesel industry. Preprints 2024, 2024021640. https://doi.org/10.20944/preprints202402.1640.v1 Carvajal, O.C.; Montes de Oca, J.M.G.; Moreno, D.C. Fermentative utilization of glycerol by E. coli and its implications in biodiesel industry. Preprints 2024, 2024021640. https://doi.org/10.20944/preprints202402.1640.v1

Abstract

The development of biofuels and biodiesel industry specifically, has resulted in the generation of large amounts of glycerol as a surplus. The conversion of glycerol, into chemicals with indus-trial interest, through anaerobic fermentation using E. coli as a biocatalyst is an attractive alter-native to improve economic performance in the biodiesel industry, for example through the bi-otransformation of residual glycerol into ethanol. For 2019, the size of the global ethanol market was valued at $ 89.1 billion US dollar. Biodiesel can be produced from several materials like rapeseed, palm, soya, corn, palm kernel, frying oil or olive-pomace oil (OPO). The last, can be extracted from alperujo, byproduct of olive oil process. However, olive-pomace oil converting into biodiesel is not enough established to achieve a global olive-oil/biodiesel derived process with minimized waste generation. Traditionally biodiesel production process produces an equivalent of 10% by mass of glycerol as a waste or by-product. This work explores the conver-sion of residual glycerol into chemicals like ethanol and hydrogen with a focus on projecting raw material potential availability from OPO, contributing to the promotion of the use of biofu-els and biodegradable compounds.

Keywords

Glycerol; Pilot scale; olive pomace oil; Fed-batch culture; Escherichia coli MG1655; Ethanol

Subject

Engineering, Chemical Engineering

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0


×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.