Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

The Use of Various Methods in Assessing the Quality of Honey in Terms of the Presence of 5-Hydroxymethylfurfural (Hmf)

Version 1 : Received: 21 February 2024 / Approved: 22 February 2024 / Online: 23 February 2024 (08:31:53 CET)

How to cite: Zembrzuska, J.; Pakulski, Ł.; Karbowska, B.; Bartoszewicz, J.; Janeba-Bartoszewicz, E.; Selech, J.; Kurczewski, P. The Use of Various Methods in Assessing the Quality of Honey in Terms of the Presence of 5-Hydroxymethylfurfural (Hmf). Preprints 2024, 2024021297. https://doi.org/10.20944/preprints202402.1297.v1 Zembrzuska, J.; Pakulski, Ł.; Karbowska, B.; Bartoszewicz, J.; Janeba-Bartoszewicz, E.; Selech, J.; Kurczewski, P. The Use of Various Methods in Assessing the Quality of Honey in Terms of the Presence of 5-Hydroxymethylfurfural (Hmf). Preprints 2024, 2024021297. https://doi.org/10.20944/preprints202402.1297.v1

Abstract

The 5-hydroxymethylfurfural (HMF) is a six-carbon heterocyclic organic compound with two substituents an aldehyde group and an alcohol group. It is formed from saccharides as a result of two reactions: the Maillard reaction and the caramelization of saccharides. It has both a negative and a positive effect on the human body, which makes it, even more, an attractive research object. The objective of the study was to determine HMF content in honey samples available on the Polish market using three varied methods and compare the results obtained. The methodology used included: two spectrophotometric methods (by White and Winkler) and the HMF determination method based on the LC-MS/MS technique developed uniquely for this study. The determined HMF content in all tested honey samples did not exceed the applicable standard. All results are within the scope of the European Council Directive 2001/110/EC of 20 December 2001, i.e. they do not exceed HMF content in honey, which is 40 mg/kg.

Keywords

5-hydroxymethylfurfural; saccharides; spectrophotometric methods; LC-MS/MS methods

Subject

Chemistry and Materials Science, Analytical Chemistry

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