Chen, X.; Wang, Y.; Han, G.; Fan, J.; Tan, Q.; Liu, G.; Zhang, H.; Wang, Y. Identification and Transfer of a New Powdery Mildew Resistance Gene PmCAHM from Landrace Changanhongmai into Common Wheat. Agronomy2024, 14, 667.
Chen, X.; Wang, Y.; Han, G.; Fan, J.; Tan, Q.; Liu, G.; Zhang, H.; Wang, Y. Identification and Transfer of a New Powdery Mildew Resistance Gene PmCAHM from Landrace Changanhongmai into Common Wheat. Agronomy 2024, 14, 667.
Chen, X.; Wang, Y.; Han, G.; Fan, J.; Tan, Q.; Liu, G.; Zhang, H.; Wang, Y. Identification and Transfer of a New Powdery Mildew Resistance Gene PmCAHM from Landrace Changanhongmai into Common Wheat. Agronomy2024, 14, 667.
Chen, X.; Wang, Y.; Han, G.; Fan, J.; Tan, Q.; Liu, G.; Zhang, H.; Wang, Y. Identification and Transfer of a New Powdery Mildew Resistance Gene PmCAHM from Landrace Changanhongmai into Common Wheat. Agronomy 2024, 14, 667.
Abstract
Powdery mildew is a severe disease causing substantial yield losses in wheat production worldwide. The Chinese wheat landrace Changanhongmai (CAHM) exhibits high resistance to the physiological race E09 of powdery mildew. This study aims to characterize the powdery mildew resistance gene in CAHM, and develop molecular markers for marker-assisted selection of wheat. To investigate the genetic characteristics of this resistant gene, we developed F1 plants, F2 generation population, and F2:3 families from a cross between CAHM and SY225. Genetic analysis demonstrated that all F1 plants were resistant to the disease, while the ratio of resistant to susceptible plants was 3: 1 in both the F2 population and F2:3 families, indicating that CAHM is inherited in a manner of single dominant powdery mildew resistance gene, which was tentatively designated as PmCAHM. By using bulk segregation analysis, we constructed a genetic map encompassing Xgwm273, Xwmc626, Xgwm11, Xgwm18, Xgdm28, Xgpw7812, Xgpw5195, Xwmc694 and PmCAHM. Among these markers, Xgpw7812 and Xgpw5195 were flanking markers tightly linked to PmCAHM at a genetic distance of 2.5 cM and 8.4 cM, respectively. Furthermore, nullisomic-tetrasomic analysis revealed that PmCAHM is located on chromosome 1B. These results indicate that PmCAHM differs from the internationally recognized powdery mildew resistance genes in both location and source. In addition, a new germplasm/line NW1748 with large seeds and powdery mildew resistance was developed from the cross and back cross populations of Fengyou 1718 (FY1718)/CAHM//FY 1718. Therefore, PmCAHM can serve as a novel powdery mildew resistance source for breeding of wheat by using NW1748 as the donor in the future.
Keywords
common wheat; powdery mildew; molecular marker; genetic population
Subject
Biology and Life Sciences, Agricultural Science and Agronomy
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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