Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Identification of Functional Genetic Variants in B4GALNT2 Gene and Their Association with Growth Traits in Goat

Liang Xu
,
Zitong Chen
,
Shuheng Chen
,
Yu Chen
,
Jiazhong Guo
ORCID logo ,
Tao Zhong
ORCID logo ,
Linjie Wang
ORCID logo ,
Siyuan Zhan
ORCID logo ,
Li Li
ORCID logo ,
Hongping Zhang
* ORCID logo ,
Jiaxue Cao
* ORCID logo
Version 1 : Received: 19 February 2024 / Approved: 19 February 2024 / Online: 19 February 2024 (13:27:54 CET)

A peer-reviewed article of this Preprint also exists.

Xu, L.; Chen, Z.; Chen, S.; Chen, Y.; Guo, J.; Zhong, T.; Wang, L.; Zhan, S.; Li, L.; Zhang, H.; Cao, J. An Identification of Functional Genetic Variants in B4GALNT2 Gene and Their Association with Growth Traits in Goats. Genes 2024, 15, 330. Xu, L.; Chen, Z.; Chen, S.; Chen, Y.; Guo, J.; Zhong, T.; Wang, L.; Zhan, S.; Li, L.; Zhang, H.; Cao, J. An Identification of Functional Genetic Variants in B4GALNT2 Gene and Their Association with Growth Traits in Goats. Genes 2024, 15, 330.

Abstract

B4GALNT2 is a vital candidate gene that affects the growth traits litter size in and growth traits of sheep, but whether it has the same function in goats remains to be further investigated. In this study, we utilized 348 Nanjiang Yellow goats as experimental subjects, conducted a whole exon, and the conserved the non-coding region SNP scanning of the B4GALNT2 gene. Our results revealed the presence of a synonymous mutation, rs672215506, within the exon of the B4GALNT2 gene in the Nanjiang Yellow goat population. This mutation was found to be associated with reduced mRNA stability of the B4GALNT2 gene. The results of SNPs detection of conserved non-coding region of B4GALNT2 gene showed that there were five potential regulatory SNPs in the Nanjiang Yellow goat population, except for rs66095343, the ~500 bp fragments of four SNPs (rs649127714, rs649573228, rs652899012 and rs639183528) could significantly increase luciferase activity both in goat skeletal muscle cells and 293T cells . Genetic diversity indexes indicated low or intermediate levels for all six SNPs analyzed, each conforming to Hardy-Weinberg equilibrium regarding genotypic frequencies. Association analysis indicated that rs660965343, rs649127714 and rs649573228 were significantly correlated with growth traits in the later stage of growth and development of Nanjiang Yellow goat. The haplotype combinations of H2H3 and H2H2 had higher body weight and greater body size; moreover, H2H2 haplotype combinations were significantly correlated with the litter size of Nanjiang Yellow goat. The results of our study demonstrate the potential utility of the B4GALNT2 gene as a functional genetic marker in breeding programs for Nanjiang Yellow goats.

Keywords

B4GALNT2; SNP; Association analysis; Goat; Growth traits

Subject

Biology and Life Sciences, Animal Science, Veterinary Science and Zoology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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