VALENCIA-LOZANO, E.; BARRAZA-CELIS, A.; IBARRA-RENDON, J.; CABRERA-PONCE, J.L. Transcriptome Analysis of Coffee Coffea arabica L. Drought Tolerant Somatic Embryogenic Line, Mediated by Antisense Trehalase. Preprints2024, 2024020961. https://doi.org/10.20944/preprints202402.0961.v1
APA Style
VALENCIA-LOZANO, E., BARRAZA-CELIS, A., IBARRA-RENDON, J., & CABRERA-PONCE, J.L. (2024). Transcriptome Analysis of Coffee Coffea arabica L. Drought Tolerant Somatic Embryogenic Line, Mediated by Antisense Trehalase. Preprints. https://doi.org/10.20944/preprints202402.0961.v1
Chicago/Turabian Style
VALENCIA-LOZANO, E., JORGE IBARRA-RENDON and JOSE LUIS CABRERA-PONCE. 2024 "Transcriptome Analysis of Coffee Coffea arabica L. Drought Tolerant Somatic Embryogenic Line, Mediated by Antisense Trehalase" Preprints. https://doi.org/10.20944/preprints202402.0961.v1
Abstract
Coffee Coffea arabica L. depends on abundantly distributed rainfall and drought impacts their development, fruit production, bean quality, and ultimately, beverage quality.
Plant Biotechnology by means of genetic manipulation and plant regeneration by the somatic embryogenic process is an alternative technology to overcome these prob-lems. In the present work we used the molecular approach of the trehalose/trehalase role in plant surviving in extreme drought/salt environments.
We used a cassette containing the antisense C. arabica L. trehalase gene under the RD29 promoter from A. thaliana and the NOS terminator to genetically modified an em-bryogenic coffee C. arabica L. cv typica line under osmotic stress supplemented with mannitol (0.3M) and sorbitol (0.3M) containing medium.
Drought resistant SE lines were recovered and regenerate plants. Resistant SE lines showed a higher rate of competence to induce secondary SE capacity. A transcrip-tome analysis was performed from high competent SE lines to understand the acquired molecular mechanisms to developed SE.
From the up-regulated genes, a PPI network made by STRING v12.0 with high confidence (0.700) revealed the presence of the 10 modules. The cell cycle, chromatin remodeling, somatic embryogenesis, oxidative stress, generic transcription pathway, carbon metabolism, phenylpropanoid biosynthesis, trehalose biosynthesis, proline bio-synthesis and glycerolipid metabolism.
Biology and Life Sciences, Biochemistry and Molecular Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
