Version 1
: Received: 10 February 2024 / Approved: 12 February 2024 / Online: 12 February 2024 (11:10:20 CET)
How to cite:
Che-Kamaruddin, N.; Md Isa, N.M. Comparative Sensitivity Between Fecal Sedimentation And Fecal Antigen Elisa For Diagnosis Of Fasciola Infection In Cattle. Preprints2024, 2024020678. https://doi.org/10.20944/preprints202402.0678.v1
Che-Kamaruddin, N.; Md Isa, N.M. Comparative Sensitivity Between Fecal Sedimentation And Fecal Antigen Elisa For Diagnosis Of Fasciola Infection In Cattle. Preprints 2024, 2024020678. https://doi.org/10.20944/preprints202402.0678.v1
Che-Kamaruddin, N.; Md Isa, N.M. Comparative Sensitivity Between Fecal Sedimentation And Fecal Antigen Elisa For Diagnosis Of Fasciola Infection In Cattle. Preprints2024, 2024020678. https://doi.org/10.20944/preprints202402.0678.v1
APA Style
Che-Kamaruddin, N., & Md Isa, N.M. (2024). Comparative Sensitivity Between Fecal Sedimentation And Fecal Antigen Elisa For Diagnosis Of Fasciola Infection In Cattle. Preprints. https://doi.org/10.20944/preprints202402.0678.v1
Chicago/Turabian Style
Che-Kamaruddin, N. and Nur Mahiza Md Isa. 2024 "Comparative Sensitivity Between Fecal Sedimentation And Fecal Antigen Elisa For Diagnosis Of Fasciola Infection In Cattle" Preprints. https://doi.org/10.20944/preprints202402.0678.v1
Abstract
The detection of Fasciola eggs in ruminant fecal samples typically occurs around 15 weeks post-infection during patent infections. The recent introduction of coproantigen ELISA (cELISA) diagnostics, which capture antigenic enzymes in fecal material, holds promise for monitoring Fasciola fecal egg count (FFEC) to implement control programs, particularly targeted treatments. This study compared two study groups, those with positive FFEC (FFEC+ve) and negative FFEC (FFEC-ve), to assess the sensitivity of Flukefinder sedimentation and a cELISA kit. This study reports that the coproantigen ELISA detection limit was 4.5 eggs per gram (epg) for a 100% positivity rate. Additionally, a moderate, statistically significant positive correlation (r²=0.716, p-value < 0.01) and an odds ratio of 1.96 (p-value < 0.01) were observed between FFEC and Fasciola coproantigen from cELISA. This implies that for every single unit increase in FFEC, there is a 96% likelihood of obtaining a higher optical density (OD) reading from the cELISA test. The results suggest that cELISA can complement fecal sedimentation tests, serving as a valuable tool for detecting cattle with high FFEC. This capability facilitates targeted treatment strategies, thereby aiding in the control and prevention of the spread of fasciolosis within the studied area.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
