PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Prolonged Oral Administration of Ethyl Alcohol Leads to Histopathology of the Epididymis and Seminal Vesicle and Changes of Metabolite Composition in the Tissue Lumen
Version 1
: Received: 8 February 2024 / Approved: 9 February 2024 / Online: 9 February 2024 (12:37:11 CET)
How to cite:
Taoto, C.; Tangsrisakda, N.; Thukhammee, W.; Iamsaard, S.; Tanphaichitr, N. Prolonged Oral Administration of Ethyl Alcohol Leads to Histopathology of the Epididymis and Seminal Vesicle and Changes of Metabolite Composition in the Tissue Lumen. Preprints2024, 2024020594. https://doi.org/10.20944/preprints202402.0594.v1
Taoto, C.; Tangsrisakda, N.; Thukhammee, W.; Iamsaard, S.; Tanphaichitr, N. Prolonged Oral Administration of Ethyl Alcohol Leads to Histopathology of the Epididymis and Seminal Vesicle and Changes of Metabolite Composition in the Tissue Lumen. Preprints 2024, 2024020594. https://doi.org/10.20944/preprints202402.0594.v1
Taoto, C.; Tangsrisakda, N.; Thukhammee, W.; Iamsaard, S.; Tanphaichitr, N. Prolonged Oral Administration of Ethyl Alcohol Leads to Histopathology of the Epididymis and Seminal Vesicle and Changes of Metabolite Composition in the Tissue Lumen. Preprints2024, 2024020594. https://doi.org/10.20944/preprints202402.0594.v1
APA Style
Taoto, C., Tangsrisakda, N., Thukhammee, W., Iamsaard, S., & Tanphaichitr, N. (2024). Prolonged Oral Administration of Ethyl Alcohol Leads to Histopathology of the Epididymis and Seminal Vesicle and Changes of Metabolite Composition in the Tissue Lumen. Preprints. https://doi.org/10.20944/preprints202402.0594.v1
Chicago/Turabian Style
Taoto, C., Sitthichai Iamsaard and Nongnuj Tanphaichitr. 2024 "Prolonged Oral Administration of Ethyl Alcohol Leads to Histopathology of the Epididymis and Seminal Vesicle and Changes of Metabolite Composition in the Tissue Lumen" Preprints. https://doi.org/10.20944/preprints202402.0594.v1
Abstract
Prolonged ethanol (EtOH) consumption is associated with male infertility with a decreased spermatogenesis rate as one cause. Defective maturation and development of sperm during their storage in the cauda epididymis and transit in the seminal vesicle can be another cause, possibly occurring before the drastic spermatogenesis disruption. Herein, we demonstrated that the cauda epididymis and seminal vesicle of rats, orally fed with EtOH under a regimen that spermatogenesis was still ongoing, showed histological damage, including lesion and decreased height of the epithelial cells, and increased collagen fibers in the muscle layer, which implicated fibrosis. Lipid peroxidation (shown by malondialdehyde (MDA) levels) was observed, indicating that reactive oxygen species (ROS) was produced along with acetaldehyde during EtOH metabolism by CYP2E1. MDA, acetaldehyde and other lipid peroxidation products could further damage cellular components of the cauda epididymis and seminal vesicle and this was supported by increased apoptosis (shown by TUNEL assay and caspase 9/caspase 3 expression) in these two tissues of EtOH-treated rats. Consequently, functionality of the cauda epididymis and seminal vesicle in EtOH-treated rats was impaired, as demonstrated by decreases in 1H NMR-analyzed metabolites (e.g., carnitine, fructose), which were important for sperm development, metabolism and survival in their lumen.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
