Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Mir-21 Regulates Growth and Migration of Cervical Cancer Cells by RECK Signaling Pathway

Seidy Y. Aguilar-Martínez
,
Gabriela E. Campos-Viguri
,
Selma E. Medina-García
,
Ricardo J. García-Flores
,
Jessica Deas
,
Claudia Gómez-Cerón
,
Abraham Pedroza-Torres
ORCID logo ,
Elizabeth Bautista-Rodríguez
,
Gloria Fernández-Tilapa
,
Mauricio Rodríguez-Dorantes
,
Carlos Pérez-Plasencia
ORCID logo ,
Oscar Peralta-Zaragoza
*
Version 1 : Received: 8 February 2024 / Approved: 9 February 2024 / Online: 9 February 2024 (11:20:36 CET)

A peer-reviewed article of this Preprint also exists.

Aguilar-Martínez, S.Y.; Campos-Viguri, G.E.; Medina-García, S.E.; García-Flores, R.J.; Deas, J.; Gómez-Cerón, C.; Pedroza-Torres, A.; Bautista-Rodríguez, E.; Fernández-Tilapa, G.; Rodríguez-Dorantes, M.; Pérez-Plasencia, C.; Peralta-Zaragoza, O. MiR-21 Regulates Growth and Migration of Cervical Cancer Cells by RECK Signaling Pathway. Int. J. Mol. Sci. 2024, 25, 4086. Aguilar-Martínez, S.Y.; Campos-Viguri, G.E.; Medina-García, S.E.; García-Flores, R.J.; Deas, J.; Gómez-Cerón, C.; Pedroza-Torres, A.; Bautista-Rodríguez, E.; Fernández-Tilapa, G.; Rodríguez-Dorantes, M.; Pérez-Plasencia, C.; Peralta-Zaragoza, O. MiR-21 Regulates Growth and Migration of Cervical Cancer Cells by RECK Signaling Pathway. Int. J. Mol. Sci. 2024, 25, 4086.

Abstract

Expression of miR-21 has been found to be altered in almost all types of cancers and it has been classified as an oncogenic microRNA. In addition, expression of tumor suppressor gene RECK is associated with miR-21 overexpression in high-grade cervical lesions. In the present study we analyze the role of miR-21 in RECK gene regulation in cervical cancer cells. To identify the downstream cellular target genes of upstream miR-21, we silenced endogenous miR-21 expression using siRNAs. We analyzed the expression of miR-21 and RECK, as well as functional effects on cell proliferation and migration. We found that in cervical cancer cells there was an inverse correlation between miR-21 expression and RECK mRNA and protein expression. SiRNAs to miR-21 increased luciferase reporter activity in construct plasmids containing the RECK-3′-UTR microRNA response elements MRE21-1, MRE21-2, and MRE21-3. The role of miR-21 in cell proliferation was also analyzed and cancer cells transfected with siRNAs exhibited a markedly reduced cell proliferation and migration. Our findings indicate that miR-21 post-transcriptionally down-regulates the expression of RECK to promote cell proliferation and cell migration inhibition in cervical cancer cell survival. Therefore, miR-21 and RECK may be potential therapeutic targets in gene therapy for cervical cancer.

Keywords

cervical cancer; microRNAs; miR-21; RECK; siRNAs

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0
Get PDF Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.