Version 1
: Received: 6 February 2024 / Approved: 7 February 2024 / Online: 7 February 2024 (14:15:59 CET)
How to cite:
Ahmed, M.Y.; Salman, N.A.; Cakir, M.O.; Sandhu, S.; Ashrafi, G.H. Concurrent High Risk HPV35, HPV45, and HPV59 Infections in Prostate and Bladder Cancer Tissues of a Single Patient. Preprints2024, 2024020451. https://doi.org/10.20944/preprints202402.0451.v1
Ahmed, M.Y.; Salman, N.A.; Cakir, M.O.; Sandhu, S.; Ashrafi, G.H. Concurrent High Risk HPV35, HPV45, and HPV59 Infections in Prostate and Bladder Cancer Tissues of a Single Patient. Preprints 2024, 2024020451. https://doi.org/10.20944/preprints202402.0451.v1
Ahmed, M.Y.; Salman, N.A.; Cakir, M.O.; Sandhu, S.; Ashrafi, G.H. Concurrent High Risk HPV35, HPV45, and HPV59 Infections in Prostate and Bladder Cancer Tissues of a Single Patient. Preprints2024, 2024020451. https://doi.org/10.20944/preprints202402.0451.v1
APA Style
Ahmed, M.Y., Salman, N.A., Cakir, M.O., Sandhu, S., & Ashrafi, G.H. (2024). Concurrent High Risk HPV35, HPV45, and HPV59 Infections in Prostate and Bladder Cancer Tissues of a Single Patient. Preprints. https://doi.org/10.20944/preprints202402.0451.v1
Chicago/Turabian Style
Ahmed, M.Y., Sarbjinder Sandhu and G Hossein Ashrafi. 2024 "Concurrent High Risk HPV35, HPV45, and HPV59 Infections in Prostate and Bladder Cancer Tissues of a Single Patient" Preprints. https://doi.org/10.20944/preprints202402.0451.v1
Abstract
Human papillomavirus (HPV) infections, primarily transmitted through sexual contact, have been linked to various cancers, including cervical, penile, anal, oropharynx, breast, and prostate cancers. This study presents a unique case of concurrent high-risk HPV35, HPV45, and HPV59 infections in both prostate and bladder cancer tissues from a single patient, representing the first documented instance worldwide with identical HPV types detected in two adjacent organs of the same individual. Employing a multiplex-PCR approach, gel electrophoresis, and Sanger sequencing, we confirmed the presence of these high-risk HPV types. Additionally, Western blot analysis using an HPV E7 antibody demonstrated the active expression of HPV oncoproteins in both cancer types. This discovery underscores the potential for HPV intra-organ transmission and necessitates further exploration of alternative transmission routes. The implications of our results offer new insights into the complex dynamics of HPV transmission in cancer pathogenesis.
Keywords
Human papillomavirus (HPV); Concurrent infections; Urogenital cancers; High-risk HPV types; Prostate cancer; Bladder cancer
Subject
Biology and Life Sciences, Virology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
