Truchado, D.A.; Juárez-Molina, M.; Rincón, S.; Zurita, L.; Tomé-Amat, J.; Lorz, C.; Ponz, F. A Multifunctionalized Potyvirus-Derived Nanoparticle That Targets and Internalizes into Cancer Cells. Int. J. Mol. Sci.2024, 25, 4327.
Truchado, D.A.; Juárez-Molina, M.; Rincón, S.; Zurita, L.; Tomé-Amat, J.; Lorz, C.; Ponz, F. A Multifunctionalized Potyvirus-Derived Nanoparticle That Targets and Internalizes into Cancer Cells. Int. J. Mol. Sci. 2024, 25, 4327.
Truchado, D.A.; Juárez-Molina, M.; Rincón, S.; Zurita, L.; Tomé-Amat, J.; Lorz, C.; Ponz, F. A Multifunctionalized Potyvirus-Derived Nanoparticle That Targets and Internalizes into Cancer Cells. Int. J. Mol. Sci.2024, 25, 4327.
Truchado, D.A.; Juárez-Molina, M.; Rincón, S.; Zurita, L.; Tomé-Amat, J.; Lorz, C.; Ponz, F. A Multifunctionalized Potyvirus-Derived Nanoparticle That Targets and Internalizes into Cancer Cells. Int. J. Mol. Sci. 2024, 25, 4327.
Abstract
Plant viral nanoparticles (VNPs) are attractive to researchers in nanomedicine due to their safety, easy production, resistance, and straightforward functionalization. In this paper, we developed and successfully purified a VNP derived from turnip mosaic virus (TuMV), a well-known plant pathogen, that exhibits high affinity for immunoglobulins G (IgG) thanks to its functionalization with the Z domain from staphylococcal protein A via gene fusion. We selected cetuximab as a model IgG to prove the versatility of this novel TuMV VNP by developing a fluorescent nanoplatform to mark tumoral cells of the line Cal33, from tongue squamous cell carcinoma. We observed that the fluorescent VNP-cetuximab bound selectively to Cal33 and were internalized by confocal microscopy, revealing the potential of this nanotool in cancer research.
Biology and Life Sciences, Biology and Biotechnology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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