Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

A piRNA Portrait for Blood Cells of Healthy Donors and COVID-19 Patients

Kirill Kondratov
* ORCID logo ,
Alexander Andreevich Artamonov
ORCID logo ,
Yuri Nikitin
ORCID logo ,
Anastasiya Velmiskina
,
Vladimir Mikhailovskii
ORCID logo ,
Sergei Mosenko
ORCID logo ,
Irina Polkovnikova
,
Anna Asinovskaya
,
Svetlana Apalko
,
Natalya Sushentseva
,
Andrey Ivanov
,
Sergey Shcherbak
Version 1 : Received: 6 February 2024 / Approved: 6 February 2024 / Online: 6 February 2024 (15:21:22 CET)

How to cite: Kondratov, K.; Artamonov, A.A.; Nikitin, Y.; Velmiskina, A.; Mikhailovskii, V.; Mosenko, S.; Polkovnikova, I.; Asinovskaya, A.; Apalko, S.; Sushentseva, N.; Ivanov, A.; Shcherbak, S. A piRNA Portrait for Blood Cells of Healthy Donors and COVID-19 Patients. Preprints 2024, 2024020379. https://doi.org/10.20944/preprints202402.0379.v1 Kondratov, K.; Artamonov, A.A.; Nikitin, Y.; Velmiskina, A.; Mikhailovskii, V.; Mosenko, S.; Polkovnikova, I.; Asinovskaya, A.; Apalko, S.; Sushentseva, N.; Ivanov, A.; Shcherbak, S. A piRNA Portrait for Blood Cells of Healthy Donors and COVID-19 Patients. Preprints 2024, 2024020379. https://doi.org/10.20944/preprints202402.0379.v1

Abstract

The expression of non-coding RNAs varies greatly depending on the cell type and physiological state. Changes in expression affect the regulatory properties of these molecules. We investigated RNA-seq data from sorted blood cells of healthy control donors and severe COVID-19 patients. Our aim was to display a distinguishable piRNA differential expression portrait for each type of cell and to study the behavior of piRNA in the blood cells of COVID-19 patients. Overall piRNA expression in sorted control cells (erythrocytes, monocytes, lymphocytes, eosinophils, basophils, neutrophils) differs depending on cell type. Four of the most abundant piRNA were expressed relatively equally in all cell types, they included piR-49145, piR-49144, piR-49143 and piR-33151. By reviewing piRNA expression in each group of sorted cells from severe COVID-19 patients we observed 3 upregulated and 10 downregulated piRNAs in erythrocytes. Monocytes were presented with a larger amount of statistically significant piRNA, 4 upregulated and 35 downregulated. In lymphocytes, all 19 piRNAs were upregulated.

Keywords

piRNA; blood cells; severe COVID-19; erythrocytes; monocytes; lymphocytes; next generation sequencing of smallRNAs; eosinophils; basophils; neutrophils

Subject

Biology and Life Sciences, Biochemistry and Molecular Biology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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