Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Multi-drug Resistant Extended-Spectrum Beta-Lactamase (ESBL)-Producing Escherichia coli in a Dairy Herd: Distribution and Antimicrobial Resistance Profiles

Martina Penati
ORCID logo ,
Laura Musa
,
Laura Filippone Pavesi
,
Alessandro Guaraglia
ORCID logo ,
Fernando Ulloa
ORCID logo ,
Paolo Moroni
ORCID logo ,
Renata Piccinini
,
M. Filippa Addis
* ORCID logo
Version 1 : Received: 1 February 2024 / Approved: 2 February 2024 / Online: 2 February 2024 (14:20:05 CET)

A peer-reviewed article of this Preprint also exists.

Penati, M.; Musa, L.; Filippone Pavesi, L.; Guaraglia, A.; Ulloa, F.; Moroni, P.; Piccinini, R.; Addis, M.F. Multidrug-Resistant Extended-Spectrum Beta-Lactamase (ESBL)-Producing Escherichia coli in a Dairy Herd: Distribution and Antimicrobial Resistance Profiles. Antibiotics 2024, 13, 241. Penati, M.; Musa, L.; Filippone Pavesi, L.; Guaraglia, A.; Ulloa, F.; Moroni, P.; Piccinini, R.; Addis, M.F. Multidrug-Resistant Extended-Spectrum Beta-Lactamase (ESBL)-Producing Escherichia coli in a Dairy Herd: Distribution and Antimicrobial Resistance Profiles. Antibiotics 2024, 13, 241.

Abstract

This study investigated the presence, distribution, and antimicrobial resistance profiles of extend-ed-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae in a dairy herd located in Northern Italy. A questionnaire was administered, and the feces of clinically healthy pre-weaned dairy calves, their mothers, and the cows treated for mastitis, waste milk, water, and environmental samples, were collected and inoculated on Chromagar ESBL™ medium. The questionnaire identi-fied several risk factors including administration of waste milk to male calves, lack of routine cleaning of calf feeding and housing equipment, and blanket dry cow therapy. Escherichia coli was identified by MALDI-TOF MS in the feces of 28 of 37 (75.67%) calves, 2 of 3 (66.67%) treated cows, 8 of 14 (57.15%) environmental samples, and the waste milk. Six of 14 (42.85%) environmental samples were also positive for ESBL Acinetobacter baumannii, while all fecal samples were negative. ESBL production was confirmed for all E. coli isolates based on the double disk susceptibility test (DDST), while none were carbapenemase producers. Upon minimal inhibitory concentration (MIC) analysis, all of them showed multiple resistances and were categorized as multi-drug resistant (MDR). This study confirms the widespread presence of MDR, ESBL-E. coli in dairy calves and highlights their diffusion in the farm environment.

Keywords

antimicrobial resistance; biosecurity; dairy calves; ESBL E. coli; multi-drug resistant; waste milk

Subject

Biology and Life Sciences, Animal Science, Veterinary Science and Zoology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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