Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Gut Microbiota Signatures with Potential Clinical Usefulness in Colorectal and Non-small Cell Lung Cancers

Sofía Tesolato
,
Juan Vicente-Valor
ORCID logo ,
Mateo Paz-Cabezas
,
Dulcenombre Gómez-Garre
ORCID logo ,
Silvia Sánchez-González
ORCID logo ,
Adriana Ortega-Hernández
,
Sofía Cristina De la Serna
ORCID logo ,
Inmaculada Domínguez-Serrano
,
Jana Dziakova
,
Daniel Rivera-Alonso
,
José Ramón Jarabo-Sarceda
,
Florentino Hernando Trancho
,
Ana maria gomez Martinez
,
Antonio José Torres-García
,
Pilar Iniesta
* ORCID logo
Version 1 : Received: 31 January 2024 / Approved: 1 February 2024 / Online: 1 February 2024 (08:28:41 CET)

A peer-reviewed article of this Preprint also exists.

Tesolato, S.; Vicente-Valor, J.; Paz-Cabezas, M.; Gómez-Garre, D.; Sánchez-González, S.; Ortega-Hernández, A.; de la Serna, S.; Domínguez-Serrano, I.; Dziakova, J.; Rivera, D.; Jarabo, J.-R.; Gómez-Martínez, A.-M.; Hernando, F.; Torres, A.; Iniesta, P. Gut Microbiota Signatures with Potential Clinical Usefulness in Colorectal and Non-Small Cell Lung Cancers. Biomedicines 2024, 12, 703. Tesolato, S.; Vicente-Valor, J.; Paz-Cabezas, M.; Gómez-Garre, D.; Sánchez-González, S.; Ortega-Hernández, A.; de la Serna, S.; Domínguez-Serrano, I.; Dziakova, J.; Rivera, D.; Jarabo, J.-R.; Gómez-Martínez, A.-M.; Hernando, F.; Torres, A.; Iniesta, P. Gut Microbiota Signatures with Potential Clinical Usefulness in Colorectal and Non-Small Cell Lung Cancers. Biomedicines 2024, 12, 703.

Abstract

The application of bacterial metagenomic analysis as biomarker for cancer detection is emerging. Our aim was to discover gut microbiota signatures with potential utility in the diagnosis of colorectal cancer (CRC) and non-small cell lung cancer (NSCLC). A prospective study was performed on a total of 77 fecal samples from CRC and NSCLC patients and Controls. DNA from stool was analyzed for bacterial genomic sequencing using the Ion Torrent™ technology. Bioinformatic analysis was performed with QIIME2 pipeline. We applied logistic regression for adjusting differences attributable to sex, age and body mass index and compared diagnostic accuracy of our gut signatures with other previously published. The feces of patients affected by different tumor types, such as CRC and NSCLC, showed a differential intestinal microbiota profile. After adjusting for confounders, Parvimonas (OR = 53.3), Gemella (OR = 6.01), Eisenbergiella (OR = 5.35), Peptostreptococcus (OR = 9.42), Lactobacillus (OR = 6.72), Salmonella (OR = 5.44) and Fusobacterium (OR = 78.9) remained significantly associated with the risk of CRC. Two genera from the Ruminoccocaeae family, DTU089 (OR = 20.1) and an uncharacterized genus (OR = 160.1) were associated with the risk of NSCLC. Our two panels had better diagnostic capacity for CRC (AUC = 0.840) and NSLC (AUC = 0.747) compared to the application to our population of two other published panels. Thus, we propose a gut bacteria panel for each cancer type and show its potential application in cancer diagnosis.

Keywords

Microbiota; Biomarker; Colorectal Cancer; Non-Small Cell Lung Cancer

Subject

Biology and Life Sciences, Life Sciences

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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