Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Mechanism of the Anti-obesity Effects of a Standardized Brassica juncea Extract in 3T3-L1 Preadipocytes and High-Fat Diet-Induced C57BL/6J Mice

June seok Lim
ORCID logo ,
Ji-Hyun Im
,
Xiaoggao Han
,
Xiao Men
,
Geon Oh
ORCID logo ,
XioLu Fu
,
Woon Sang Hwang
,
Sun-Il Choi
,
Ok-Hwan Lee
*
Version 1 : Received: 25 January 2024 / Approved: 25 January 2024 / Online: 25 January 2024 (14:24:23 CET)

A peer-reviewed article of this Preprint also exists.

Lim, J.-S.; Im, J.-H.; Han, X.; Men, X.; Oh, G.; Fu, X.; Hwang, W.; Choi, S.-I.; Lee, O.-H. The Mechanism of the Anti-Obesity Effects of a Standardized Brassica juncea Extract in 3T3-L1 Preadipocytes and High-Fat Diet-Induced Obese C57BL/6J Mice. Nutrients 2024, 16, 846. Lim, J.-S.; Im, J.-H.; Han, X.; Men, X.; Oh, G.; Fu, X.; Hwang, W.; Choi, S.-I.; Lee, O.-H. The Mechanism of the Anti-Obesity Effects of a Standardized Brassica juncea Extract in 3T3-L1 Preadipocytes and High-Fat Diet-Induced Obese C57BL/6J Mice. Nutrients 2024, 16, 846.

Abstract

Obesity is a global health concern. Recent research has suggested that the development of anti-obesity ingredients and functional foods should focus on natural products without side effects. We investigated the anti-obesity efficacy and mechanisms of action of Brassica juncea extract (BJE) using in vitro and in vivo obesity models. In in vitro experiments, BJE treatment downregulated adipogenic-related proteins CCAAT/enhancer-binding protein-α (C/EBP-α), peroxisome proliferator-activated receptor-γ (PPAR-γ), adipocyte protein 2 (aP2), and lipid synthesis-related protein acetyl-CoA carboxylase (ACC). It also upregulated the heat generation protein peroxisome proliferator-activated receptor gamma coactivator-1α (PGC-1α) and fatty acid oxidation protein carnitine palmitoyltransferase-1 (CPT-1). Oral administration of BJE decreased body weight, alleviated liver damage, and inhibited lipid accumulation in high-fat diet (HFD)-induced obese mice. Inhibition of lipid accumulation by BJE in vivo was associated with decreased expression of adipogenic and lipid synthesis proteins and increased expression of heat generation and fatty acid oxidation proteins. BJE administration improved obesity by decreasing adipogenesis and activating heat generation and fatty acid oxidation in 3T3-L1 cells and in HFD-induced obese C57BL/6J mice. These findings indicate that BJE is a promising natural means of preventing obesity-related metabolic diseases.

Keywords

standardized Brassica juncea; 3T3-L1; C57BL/6J; anti-obesity; mechanism

Subject

Biology and Life Sciences, Food Science and Technology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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