Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Refinement of the rKLi8.3 Based Serodiagnostic Elisa Allows Detection of Canine Visceral Leishmaniasis in Dogs with Low Antibody Titers

Henrique Couto Teixeira
* ORCID logo ,
Giulia Pereira Costa Valle
,
Rouzbeh Mahdavi
,
Priscila Santos Martins Dias
,
Erick Esteves De Oliveira
,
Cristina Aira
ORCID logo ,
Daniela Heinz
,
Andreas Latz
,
Marta de Lana
ORCID logo ,
Fernanda Morgado
ORCID logo ,
Renato Porrozzi
,
Ulrich Steinhoff
ORCID logo
Version 1 : Received: 24 January 2024 / Approved: 24 January 2024 / Online: 24 January 2024 (14:47:56 CET)

A peer-reviewed article of this Preprint also exists.

Teixeira, H.C.; Valle, G.P.C.; Mahdavi, R.; Dias, P.S.M.; de Oliveira, E.E.; Aira, C.P.; Heinz, D.; Latz, A.; Lana, M.D.; Morgado, F.N.; Porrozzi, R.; Steinhoff, U. Refinement of the rKLi8.3-Based Serodiagnostic ELISA Allows Detection of Canine Leishmaniosis in Dogs with Low Antibody Titers. Pathogens 2024, 13, 246. Teixeira, H.C.; Valle, G.P.C.; Mahdavi, R.; Dias, P.S.M.; de Oliveira, E.E.; Aira, C.P.; Heinz, D.; Latz, A.; Lana, M.D.; Morgado, F.N.; Porrozzi, R.; Steinhoff, U. Refinement of the rKLi8.3-Based Serodiagnostic ELISA Allows Detection of Canine Leishmaniosis in Dogs with Low Antibody Titers. Pathogens 2024, 13, 246.

Abstract

Diagnosis of canine leishmaniosis (CanL) still represents a major challenge due to the variable clinical manifestations and the large number of asymptomatic dogs. Serological tests are most commonly used to detect infected animals, revealing anti-Leishmania antibodies, mainly of IgG isotype. Recently, a new diagnostic antigen, rKLi8.3, containing 8.3 kinesin tandem repeats (TR) from an Leishmania infantum strain from Sudan has been shown to provide excellent specificity and sensitivity to Leishmania infected humans and dogs. However, asymptomatic animals with very low antibody titers are often difficult to detect by serodiagnosis. Thus, we wondered whether addition of an anti-IgG-enhancing step in the protein A/G-based rKLi8.3-ELISA will improve the diagnostic performance without decreasing the specificity. For this, parasitologically confirmed CanL cases with low or high clinical score, uninfected healthy controls and dogs with other infections were tested by rKLi8.3-ELISA as well as two different immunochromatographic rapid tests, rKLi8.3-lateral flow test (LFT) and Dual Path Platform (DPP®) based on the rK28 antigen. Our results show that the diagnostic accuracy of the rKLi8.3-ELISA and LFT was similar to that of DPP, missing several asymptomatic animals. However, addition of a secondary, amplifying anti-dog IgG antibody in the protein A/G-based rKLi8.3-ELISA enabled the detection of nearly all asymptomatic dogs without affecting its specificity.

Keywords

Leishmaniasis; Canine leishmaniosis; Sero-Diagnosis; Recombinant proteins; Kinesins; POC diagnostics; Asymptomatic CanL

Subject

Biology and Life Sciences, Immunology and Microbiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF Download Supplementary

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0
Get PDF Supplementary Files Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.