Version 1
: Received: 23 January 2024 / Approved: 24 January 2024 / Online: 24 January 2024 (11:04:07 CET)
How to cite:
Halász, H.; Tárnai, V.; Matkó, J.; Nyitrai, M.; Szabó-Meleg, E. Cooperation of Various Cytoskeletal Components Orchestrates Intercellular Spread of Mitochondria between B Cells through Tunnelling Nanotubes. Preprints2024, 2024011738. https://doi.org/10.20944/preprints202401.1738.v1
Halász, H.; Tárnai, V.; Matkó, J.; Nyitrai, M.; Szabó-Meleg, E. Cooperation of Various Cytoskeletal Components Orchestrates Intercellular Spread of Mitochondria between B Cells through Tunnelling Nanotubes. Preprints 2024, 2024011738. https://doi.org/10.20944/preprints202401.1738.v1
Halász, H.; Tárnai, V.; Matkó, J.; Nyitrai, M.; Szabó-Meleg, E. Cooperation of Various Cytoskeletal Components Orchestrates Intercellular Spread of Mitochondria between B Cells through Tunnelling Nanotubes. Preprints2024, 2024011738. https://doi.org/10.20944/preprints202401.1738.v1
APA Style
Halász, H., Tárnai, V., Matkó, J., Nyitrai, M., & Szabó-Meleg, E. (2024). Cooperation of Various Cytoskeletal Components Orchestrates Intercellular Spread of Mitochondria between B Cells through Tunnelling Nanotubes. Preprints. https://doi.org/10.20944/preprints202401.1738.v1
Chicago/Turabian Style
Halász, H., Miklós Nyitrai and Edina Szabó-Meleg. 2024 "Cooperation of Various Cytoskeletal Components Orchestrates Intercellular Spread of Mitochondria between B Cells through Tunnelling Nanotubes" Preprints. https://doi.org/10.20944/preprints202401.1738.v1
Abstract
Membrane nanotubes (NTs) are dynamic communication channels connecting spatially separated cells even over long distances and promoting the transport of different cellular cargos. NTs are also involved in the intercellular spread of different pathogens and the deterioration of some neuro-logical disorders. Transport processes via NTs may be controlled by cytoskeletal elements. NTs are frequently observed membrane projections in numerous mammalian cell lines including various immune cells, but their functional significance in the ‘antibody factory’ B cells is poorly elucidated. Here we report that as active channels, NTs of B cells can mediate bidirectional mitochondrial transport, promoted by the cooperation of two different cytoskeletal motor proteins, kinesin along microtubules and myosin VI along actin, and bidirectional transport processes are also supported by the heterogeneous arrangement of the main cytoskeletal filament systems of the NTs. We revealed that despite NTs and axons being different cell extensions the mitochondrial transport they mediate may exhibit significant similarities. Furthermore, we found that microtubules may improve the stability and lifespan of B cell NTs, while F-actin strengthens NTs by providing a structural framework for them. Our results may contribute to a better understanding of the regulation of the major cells of humoral immune response to infections.
Keywords
actin; microtubule; mitochondria; transport; membrane nanotube; motor proteins
Subject
Biology and Life Sciences, Cell and Developmental Biology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
