Kokina, I.; Plaksenkova, I.; Jankovskis, L.; Jermaļonoka, M.; Galek, R. In Vivo Biosynthesis of Au and Ag NPs Using Two Medicago sativa L. Genotypes. Appl. Sci.2024, 14, 2066.
Kokina, I.; Plaksenkova, I.; Jankovskis, L.; Jermaļonoka, M.; Galek, R. In Vivo Biosynthesis of Au and Ag NPs Using Two Medicago sativa L. Genotypes. Appl. Sci. 2024, 14, 2066.
Kokina, I.; Plaksenkova, I.; Jankovskis, L.; Jermaļonoka, M.; Galek, R. In Vivo Biosynthesis of Au and Ag NPs Using Two Medicago sativa L. Genotypes. Appl. Sci.2024, 14, 2066.
Kokina, I.; Plaksenkova, I.; Jankovskis, L.; Jermaļonoka, M.; Galek, R. In Vivo Biosynthesis of Au and Ag NPs Using Two Medicago sativa L. Genotypes. Appl. Sci. 2024, 14, 2066.
Abstract
The nano size, physical and chemical properties of Au and Ag nanoparticles (NPs) allow them to be used in medicine or plant protection, but chemical solvents used during synthesis makes them toxic and pose a threat to the environment. Chemical NPs synthesis can be replaced by in vivo synthesis in which independently growing plants such as alfalfa take up and then split the precursor in their cells down to nano size using synthesis-promoting solvents–biomolecules, which can break down materials without free radicals and has anti-inflammatory, antioxidant effects, making NPs environmentally benign.
In this study, two-week-old seedlings of two Medicago sativa L. genotypes, ‘Kometa’ and ‘la Bella’, were exposed to two precursors (AgNO3, HAuCl4) for 24 and 48 h to determine whether in vivo synthesis is possible. Double-blazed and certain wavelength spectrophotometry and confocal microscopy confirmed, statistically significant (P<0.05) changes in light absorption and light fluorescence compared to the control. Confocal microscopy showed both precursors visible in the roots of both genotypes. Currently, NPs synthesis and visualisation methods require a complex, expensive and time-consuming sequence of methods. It is important to find an effective, environmentally friendly, and as cheap and simple as possible method for the biosynthesis of NPs.
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