Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Biological and Antibacterial Evaluation of a New Approach to Zirconia Implant Surfaces Modified with Mineral Trioxide Aggregate (MTA)

Beatriz Ferreira Fernandes
* ,
Neusa Silva
ORCID logo ,
Mariana Brito Da Cruz
,
Gonçalo Garret
,
Óscar Carvalho
ORCID logo ,
Filipe Silva
,
António Mata
,
Helena Francisco
ORCID logo ,
Joana Faria Marques
Version 1 : Received: 19 January 2024 / Approved: 21 January 2024 / Online: 22 January 2024 (09:47:05 CET)

A peer-reviewed article of this Preprint also exists.

Fernandes, B.F.; Silva, N.; Da Cruz, M.B.; Garret, G.; Carvalho, Ó.; Silva, F.; Mata, A.; Francisco, H.; Marques, J.F. Cell Biological and Antibacterial Evaluation of a New Approach to Zirconia Implant Surfaces Modified with MTA. Biomimetics 2024, 9, 155. Fernandes, B.F.; Silva, N.; Da Cruz, M.B.; Garret, G.; Carvalho, Ó.; Silva, F.; Mata, A.; Francisco, H.; Marques, J.F. Cell Biological and Antibacterial Evaluation of a New Approach to Zirconia Implant Surfaces Modified with MTA. Biomimetics 2024, 9, 155.

Abstract

Peri-implantitis continues to be one of the major reasons for implant failures. We propose a new approach for incorporating MTA into Zirconia implant surfaces with Nd:YAG laser and investigate the biological response of peri-implant cells as well as the microbiological response. Discs of Zirconia stabilized with Yttria and Titanium were produced according 4 tested groups: Nd:YAG laser textured Zirconia coated with MTA (Zr MTA); Nd:YAG laser textured Zirconia (Zr Textured); Zirconia and Titanium discs (Zr and Ti ). Contact profilometry was used to evaluate surface roughness. Human osteoblasts, gingival fibroblasts and S. oralis were cultured on samples. Cell adhesion and morphology, cell viability, cell differentiation markers and bacterial growth were assessed. Zr Textured roughness was significantly higher compared to all other groups. Cellular adhesion was observed at 1 day in all samples in both cell lines. Osteoblasts viability was lower in Zr MTA group, unlike fibroblasts viability was shown to be higher in Zr MTA group compared to Zr Textured group at 3 and 7 days. Osteocalcin and IL-8 release by osteoblasts were higher in Zr MTA. Zr Textured showed higher IL-8 values released by fibroblasts. No differences in S. oralis CFUs were observed between groups. The results suggest that Zirconia implant surfaces coated with MTA seems to promote fibroblasts proliferation and osteoblasts differentiation, however does not appear to have antibacterial properties.

Keywords

Mineral Trioxide Aggregate, Dental implant, Zirconia, Osteoblasts, Fibroblasts

Subject

Medicine and Pharmacology, Dentistry and Oral Surgery

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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