PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Diagnostic Efficiency of CTV and HSVd for Early and Mixed Infections Using Bioamplification in Citrus Arizona 861-S1 and RT‒PCR Compared to Direct RT‒PCR
Version 1
: Received: 18 January 2024 / Approved: 19 January 2024 / Online: 19 January 2024 (07:52:21 CET)
How to cite:
Henriquez, C.A.; Riquelme, N.; Larach, A.; Besoain, X. Diagnostic Efficiency of CTV and HSVd for Early and Mixed Infections Using Bioamplification in Citrus Arizona 861-S1 and RT‒PCR Compared to Direct RT‒PCR. Preprints2024, 2024011468. https://doi.org/10.20944/preprints202401.1468.v1
Henriquez, C.A.; Riquelme, N.; Larach, A.; Besoain, X. Diagnostic Efficiency of CTV and HSVd for Early and Mixed Infections Using Bioamplification in Citrus Arizona 861-S1 and RT‒PCR Compared to Direct RT‒PCR. Preprints 2024, 2024011468. https://doi.org/10.20944/preprints202401.1468.v1
Henriquez, C.A.; Riquelme, N.; Larach, A.; Besoain, X. Diagnostic Efficiency of CTV and HSVd for Early and Mixed Infections Using Bioamplification in Citrus Arizona 861-S1 and RT‒PCR Compared to Direct RT‒PCR. Preprints2024, 2024011468. https://doi.org/10.20944/preprints202401.1468.v1
APA Style
Henriquez, C.A., Riquelme, N., Larach, A., & Besoain, X. (2024). Diagnostic Efficiency of CTV and HSVd for Early and Mixed Infections Using Bioamplification in Citrus Arizona 861-S1 and RT‒PCR Compared to Direct RT‒PCR. Preprints. https://doi.org/10.20944/preprints202401.1468.v1
Chicago/Turabian Style
Henriquez, C.A., Alejandra Larach and Ximena Besoain. 2024 "Diagnostic Efficiency of CTV and HSVd for Early and Mixed Infections Using Bioamplification in Citrus Arizona 861-S1 and RT‒PCR Compared to Direct RT‒PCR" Preprints. https://doi.org/10.20944/preprints202401.1468.v1
Abstract
This study examined the efficiency of a combined diagnostic approach in which bioamplification of Arizona 861 S1 citron was integrated with RT‒PCR to detect Citrus tristeza virus (CTV) and Hop Stunt Viroid (HSVd) in Citrus plants. The capacity of the combined method to diagnose mixed infections of CTV and HSVd, along with early single infections of both pathogens, was assessed by evaluating the incubation periods and differential detection capabilities between the combined and direct methods. The bioamplification method exhibited high sensitivity (100%) and specificity (100%) for CTV, enabling early detection even in patients with low-titer infections compared to direct RT‒PCR. However, for HSVd, the combined method achieved 50% sensitivity in early infections, highlighting the need for additional replicates to enhance diagnostic reliability. The relative concentrations of pathogens in mixed and simple infections were evaluated; the analysis revealed no significant differences, suggesting minimal interference between CTV and HSVd. This approach allows early detection of CTV but requires further refinement for HSVd diagnosis. These findings underscore the complementary roles of the cost-effectiveness of direct RT‒PCR and the sensitivity of the combined method in implementing effective disease control strategies in citrus crops.
Biology and Life Sciences, Agricultural Science and Agronomy
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
