Preprint Communication Version 1 Preserved in Portico This version is not peer-reviewed

High Risk HPV Detection Using Anyplex™ Detection Kit in Paraffin-Embedded Tissue from Cervical Lesions

Micaela Almeida
ORCID logo ,
Vítor Caeiro
,
Diana Costa
,
Lara Silva
,
Cíntia Sousa
,
Paula Pestana
,
Sofia Campelos
ORCID logo ,
João Vale
ORCID logo ,
Ana Ramalhinho
* ORCID logo ,
José Fonseca-Moutinho
,
Luiza Breitenfeld
* ORCID logo
Version 1 : Received: 18 January 2024 / Approved: 18 January 2024 / Online: 18 January 2024 (14:55:35 CET)

How to cite: Almeida, M.; Caeiro, V.; Costa, D.; Silva, L.; Sousa, C.; Pestana, P.; Campelos, S.; Vale, J.; Ramalhinho, A.; Fonseca-Moutinho, J.; Breitenfeld, L. High Risk HPV Detection Using Anyplex™ Detection Kit in Paraffin-Embedded Tissue from Cervical Lesions. Preprints 2024, 2024011434. https://doi.org/10.20944/preprints202401.1434.v1 Almeida, M.; Caeiro, V.; Costa, D.; Silva, L.; Sousa, C.; Pestana, P.; Campelos, S.; Vale, J.; Ramalhinho, A.; Fonseca-Moutinho, J.; Breitenfeld, L. High Risk HPV Detection Using Anyplex™ Detection Kit in Paraffin-Embedded Tissue from Cervical Lesions. Preprints 2024, 2024011434. https://doi.org/10.20944/preprints202401.1434.v1

Abstract

Background: Human papillomavirus (HPV) is diagnosed in most cervical cancers and is the fourth cause of cancer worldwide. Currently, fourteen HPV types are considered carcinogenic (HPV 16/18/31/33/35/39/45/51/52/56/58/59/66/68) with different oncogenicity. Cervical lesions might be due to HR (high risk)-HPV for what is important to identify new methods to genotype cervical lesions. The main goal was to identify, for the first time, HPV-HR in cervical lesions using Anyplex™ Detection kit in paraffin-embedded tissue. (2) Methods: It was performed a retrospective study in 45 women from Academic Hospital of Cova da Beira (CHUCB). DNA extraction was performed and through Anyplex™ II HPV-HR Detection kit HPV-HR were genotyped. (3) Results: HPV-HR were identified in 38 women; HPV-16 (55,26%), HPV-18/39/56/58/59 (5,26%), HPV-31 (21,05%), HPV-35 (7,89%), HPV-51/6 (2,63%) and HPV-52 (10,53%). (4) Conclusions: Our results reflect that Anyplex™ II HPV-HR Detection kit, designed for HR-HPV genotyping of cervical cancer screening, might be properly used to HR-HPV genotyping in histology. The proposed methodology allows an easy and cheaper technique that should be used as a future cervical risk stratification. It is a strong tool to be implemented in clinical practice in order to detect HR-HPV detection in cervical lesions, contributing to a more accurate diagnosis.

Keywords

HR-HPV Detection kit; HR-HPV genotyping; Cervical Cancer

Subject

Medicine and Pharmacology, Oncology and Oncogenics

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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