PreprintArticleVersion 1Preserved in Portico This version is not peer-reviewed
Evidence of Regulating Effect of Nitrogen Level on Ultramicrostructure and Mapping Elements of Tomato (Lycopersiconesculentum Mill.) Chloroplast under Heat Stress
Version 1
: Received: 18 January 2024 / Approved: 18 January 2024 / Online: 18 January 2024 (14:02:41 CET)
How to cite:
Li, C.; Yang, Z.; Zhang, C.; Luo, J.; Wang, D.; Yan, Y. Evidence of Regulating Effect of Nitrogen Level on Ultramicrostructure and Mapping Elements of Tomato (Lycopersiconesculentum Mill.) Chloroplast under Heat Stress. Preprints2024, 2024011427. https://doi.org/10.20944/preprints202401.1427.v1
Li, C.; Yang, Z.; Zhang, C.; Luo, J.; Wang, D.; Yan, Y. Evidence of Regulating Effect of Nitrogen Level on Ultramicrostructure and Mapping Elements of Tomato (Lycopersiconesculentum Mill.) Chloroplast under Heat Stress. Preprints 2024, 2024011427. https://doi.org/10.20944/preprints202401.1427.v1
Li, C.; Yang, Z.; Zhang, C.; Luo, J.; Wang, D.; Yan, Y. Evidence of Regulating Effect of Nitrogen Level on Ultramicrostructure and Mapping Elements of Tomato (Lycopersiconesculentum Mill.) Chloroplast under Heat Stress. Preprints2024, 2024011427. https://doi.org/10.20944/preprints202401.1427.v1
APA Style
Li, C., Yang, Z., Zhang, C., Luo, J., Wang, D., & Yan, Y. (2024). Evidence of Regulating Effect of Nitrogen Level on Ultramicrostructure and Mapping Elements of Tomato (<em>Lycopersiconesculentum</em> Mill.) Chloroplast under Heat Stress. Preprints. https://doi.org/10.20944/preprints202401.1427.v1
Chicago/Turabian Style
Li, C., Di Wang and Yehan Yan. 2024 "Evidence of Regulating Effect of Nitrogen Level on Ultramicrostructure and Mapping Elements of Tomato (<em>Lycopersiconesculentum</em> Mill.) Chloroplast under Heat Stress" Preprints. https://doi.org/10.20944/preprints202401.1427.v1
Abstract
Heat stress is a serious threat to tomato production. The ultrastructure and element mapping of tomato chloroplast were investigated under different nitrogen levels to explore the regulatory effect of nitrogen on tomato chloroplast under heat stress. Experiments design two temperature treatments (25°C/15°C, as control, 40°C/30°C, as severe high-temperature, SHT ) and four nitrogen levels (0.0 kg hm-2, N1; 187.5 kg hm-2, N2; 250 kg hm-2, N3, as recommended nitrogen-application; 312.5kg hm-2 , N4) for a duration of 5 days in artificial climate chamber. The results showed that heat stress led to obvious damage to the ultrastructure of tomato chloroplast, including thylakoid stacking disorder, granum destruction, and starch grain aggregation and degradation. With the increase of nitrogen level, the ratio of long and short axes of tomato chloroplasts increased, the differentiation of chloroplasts was strengthened, the number of plastoglobuli was increased and aggregated, and the degree of damage to the ultrastructure of chloroplasts was reduced. The regulatory effect of nitrogen on the ultrastructure of tomato chloroplasts under heat stress was mainly reflected in the phosphorylation degradation process of starch granules in chloroplasts. There are obvious segmentation lines of Ca, P and Cl on both sides of the inner and outer chloroplasts, and enriched in the chloroplasts, which can outline the boundary of the chloroplasts. Element mapping analysis revealed that heat stress significantly increased the contents of calcium, phosphorus and chlorine in the chloroplast of tomato leaves, while the contents of potassium was decreased. The application of nitrogen fertilizer could significantly regulate the element distribution of tomato chloroplast under heat stress, especially the regulation of phosphorus, chlorine, calcium, magnesium. These results provide new insight into the role of nitrogen using TEM-EDX mapping in the protection of tomato chloroplast under heat stress and the mechanism of heat stress tolerance in plants.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
