Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Cold-Azurin a New Antibiofilm Protein Produced by the Antarctic Marine Bacterium Pseudomonas sp. TAE6080

Caterina D’Angelo
ORCID logo ,
Marika Trecca
,
Andrea Carpentieri
,
Marco Artini
,
Laura Selan
,
Maria Luisa Tutino
ORCID logo ,
Rosanna Papa
ORCID logo ,
And Ermenegilda Parrilli
*
Version 1 : Received: 2 January 2024 / Approved: 17 January 2024 / Online: 18 January 2024 (03:13:58 CET)

A peer-reviewed article of this Preprint also exists.

D’Angelo, C.; Trecca, M.; Carpentieri, A.; Artini, M.; Selan, L.; Tutino, M.L.; Papa, R.; Parrilli, E. Cold-Azurin, a New Antibiofilm Protein Produced by the Antarctic Marine Bacterium Pseudomonas sp. TAE6080. Mar. Drugs 2024, 22, 61. D’Angelo, C.; Trecca, M.; Carpentieri, A.; Artini, M.; Selan, L.; Tutino, M.L.; Papa, R.; Parrilli, E. Cold-Azurin, a New Antibiofilm Protein Produced by the Antarctic Marine Bacterium Pseudomonas sp. TAE6080. Mar. Drugs 2024, 22, 61.

Abstract

Bacteria can colonize and develop biofilm on a large variety of biotic and abiotic materials, it is accountable for nosocomial infections and chronic illness and consequently, it is a serious economic and public health problem. Staphylococcus epidermidis, thanks to its ability to form biofilm and colonize biomaterials, represents the most frequent causative agent involved in bio-film-associated infections of medical devices. Due to the biofilm presence, S. epidermidis infections are extremely difficult to treat and the research of new molecules able to interfere with its biofilm formation has a remarkable interest. In the present work, the attention was focused on Pseudomonas sp. TAE6080, an Antarctic marine bacterium able to produce and secrete an effective antibiofilm compound. The molecule responsible for this activity was purified by an activity-guided approach and it resulted to be a periplasmic protein, named Cold-Azurin. The Cold-Azurin was recombinantly produced in E. coli and purified. The recombinant protein was able to impair S. epidermidis attachment to the polystyrene surface and effectively prevent biofilm formation.

Keywords

Staphylococcus epidermidis; antibiofilm; Azurin; cold-adapted bacteria

Subject

Biology and Life Sciences, Biology and Biotechnology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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