Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Association Between Albumin Alterations and Renal Function in Patients With Type 2 Diabetes Mellitus

Marta Nugnes
,
Maurizio Baldassarre
ORCID logo ,
Danilo Ribichini
,
Daniele Tedesco
ORCID logo ,
Irene Capelli
,
Daniele Vetrano
ORCID logo ,
Francesca Marchignoli
ORCID logo ,
Lucia Brodosi
ORCID logo ,
Enrico Pompili
ORCID logo ,
Maria Letizia Petroni
,
Gaetano La Manna
ORCID logo ,
Giulio Marchesini
ORCID logo ,
Marina Naldi
* ORCID logo ,
Manuela Bartolini
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Version 1 : Received: 11 January 2024 / Approved: 12 January 2024 / Online: 12 January 2024 (04:05:34 CET)

A peer-reviewed article of this Preprint also exists.

Nugnes, M.; Baldassarre, M.; Ribichini, D.; Tedesco, D.; Capelli, I.; Vetrano, D.; Marchignoli, F.; Brodosi, L.; Pompili, E.; Petroni, M.L.; La Manna, G.; Marchesini, G.; Naldi, M.; Bartolini, M. Association between Albumin Alterations and Renal Function in Patients with Type 2 Diabetes Mellitus. Int. J. Mol. Sci. 2024, 25, 3168. Nugnes, M.; Baldassarre, M.; Ribichini, D.; Tedesco, D.; Capelli, I.; Vetrano, D.; Marchignoli, F.; Brodosi, L.; Pompili, E.; Petroni, M.L.; La Manna, G.; Marchesini, G.; Naldi, M.; Bartolini, M. Association between Albumin Alterations and Renal Function in Patients with Type 2 Diabetes Mellitus. Int. J. Mol. Sci. 2024, 25, 3168.

Abstract

Diabetic kidney disease (DKD) is a major cause of morbidity and mortality of type 2 diabetes mellitus (T2DM). Aim of this study was to investigate whether albumin structural alterations correlate with DKD severity and evaluate whether native and reduced albumin concentrations could complement the diagnosis of DKD. To this aim, one hundred and seventeen T2DM patients without (n=42) and with (n=75) DKD (DKD I-III upon KDIGO classification) were evaluated; total albumin concentration (tHA) was quantified by bromocresol green assay, while structural alterations were profiled assessed by liquid chromatography-high resolution mass spectrometry (LC-HRMS). The concentrations of albumin in native (eHA, effective albumin) and reduced (rHA) forms were then assessed. HRMS analyses showed a reduced relative amount of native albumin in DKD patients along with an increased abundance of altered forms, especially those bearing oxidative modifications. Accordingly, both eHA and rHA values varied along the stages of progressive renal failure, and alterations dose-dependently correlated with renal dysfunction. ROC curves analysis showed a significantly higher sensitivity and specificity of eHA and rHA with respect to tHA for the diagnosis of DKD. Importantly, at multivariable logistic regression analysis, eHA was identified as independent predictor of DKD.

Keywords

diabetic kidney disease; effective albumin; reduced albumin; structural alterations; oxidative damages; high resolution mass spectrometry

Subject

Biology and Life Sciences, Life Sciences

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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