Version 1
: Received: 7 January 2024 / Approved: 8 January 2024 / Online: 8 January 2024 (11:27:33 CET)
How to cite:
Santillán-Cortez, D.; Castell-Rodríguez, A.E.; González-Arenas, A.; Suarez-Cuenca, J.A.; Hautefeuille, M.C.A.; Pérez-Koldenkova, V.; Añorve-Bailón, D.; García, S.; Toledo Lozano, C.G.; Escamilla-Tilch, M.; Mondragón-Terán, P. Optimizing Cell Inoculation in a Microfluidic System in Order to Mimic the Hemato-Encephalic Barrier and Tumor Dynamic Cell Interaction. Preprints2024, 2024010547. https://doi.org/10.20944/preprints202401.0547.v1
Santillán-Cortez, D.; Castell-Rodríguez, A.E.; González-Arenas, A.; Suarez-Cuenca, J.A.; Hautefeuille, M.C.A.; Pérez-Koldenkova, V.; Añorve-Bailón, D.; García, S.; Toledo Lozano, C.G.; Escamilla-Tilch, M.; Mondragón-Terán, P. Optimizing Cell Inoculation in a Microfluidic System in Order to Mimic the Hemato-Encephalic Barrier and Tumor Dynamic Cell Interaction. Preprints 2024, 2024010547. https://doi.org/10.20944/preprints202401.0547.v1
Santillán-Cortez, D.; Castell-Rodríguez, A.E.; González-Arenas, A.; Suarez-Cuenca, J.A.; Hautefeuille, M.C.A.; Pérez-Koldenkova, V.; Añorve-Bailón, D.; García, S.; Toledo Lozano, C.G.; Escamilla-Tilch, M.; Mondragón-Terán, P. Optimizing Cell Inoculation in a Microfluidic System in Order to Mimic the Hemato-Encephalic Barrier and Tumor Dynamic Cell Interaction. Preprints2024, 2024010547. https://doi.org/10.20944/preprints202401.0547.v1
APA Style
Santillán-Cortez, D., Castell-Rodríguez, A.E., González-Arenas, A., Suarez-Cuenca, J.A., Hautefeuille, M.C.A., Pérez-Koldenkova, V., Añorve-Bailón, D., García, S., Toledo Lozano, C.G., Escamilla-Tilch, M., & Mondragón-Terán, P. (2024). Optimizing Cell Inoculation in a Microfluidic System in Order to Mimic the Hemato-Encephalic Barrier and Tumor Dynamic Cell Interaction. Preprints. https://doi.org/10.20944/preprints202401.0547.v1
Chicago/Turabian Style
Santillán-Cortez, D., Mónica Escamilla-Tilch and Paul Mondragón-Terán. 2024 "Optimizing Cell Inoculation in a Microfluidic System in Order to Mimic the Hemato-Encephalic Barrier and Tumor Dynamic Cell Interaction" Preprints. https://doi.org/10.20944/preprints202401.0547.v1
Abstract
Microfluidic systems offer precise control over physiological, biochemical, and mechanical stimuli in cell culture, allowing in vitro emulation of tissue or tumor microenvironments. This study aims to advance our understanding of tumor biology and contribute to personalized therapy development. Overcoming challenges in cell culture, including cell density and microfluidic device properties, is essential for this purpose. We designed a microfluidic system to facilitate the interaction of diverse cell lineages, incorporating human brain microvascular endothelial cells (HBEC5i), glioblastoma multiforme cells (U87MG), and astrocytes (ScienceCells 1800). Post-fabrication, we assessed the system's functionality and systematically evaluated cell inoculation conditions. Photographic acquisitions comprehensively captured cell culture and their interactions. The device successfully enabled assessment of cellular proliferation, invasion, and migration, supporting the growth of monolayers and tumor organoids. Importantly, each channel demonstrated independence in study processes while allowing cellular intercommunication, both physically and biochemically. In conclusion, the functional microfluidic device facilitated the study of cell migration, tumor invasion, and organoid growth, faithfully replicating biological features observed in a tumor microenvironment. This research contributes to advancing our understanding of tumor biology for the development of personalized therapies.
Biology and Life Sciences, Biology and Biotechnology
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