Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

The Resistance of Soybean Variety Heinong 84 to Apple Latent Spherical Virus Is Controlled by Two Genetic Loci

Tingshuai Ma
,
Ying Zhang
,
Yong Li
ORCID logo ,
Yu Zhao
,
Kekely Bruno Attiogbe
,
Xinyue Fan
,
Wenqian Fan
,
Jiaxing Sun
,
Yalou Luo
,
Xinwei Yu
,
Weiqin Ji
ORCID logo ,
Xiaofei Cheng
* ORCID logo ,
Xiaoyun Wu
*
Version 1 : Received: 5 January 2024 / Approved: 8 January 2024 / Online: 8 January 2024 (08:05:59 CET)

A peer-reviewed article of this Preprint also exists.

Ma, T.; Zhang, Y.; Li, Y.; Zhao, Y.; Attiogbe, K.B.; Fan, X.; Fan, W.; Sun, J.; Luo, Y.; Yu, X.; Ji, W.; Cheng, X.; Wu, X. The Resistance of Soybean Variety Heinong 84 to Apple Latent Spherical Virus Is Controlled by Two Genetic Loci. Int. J. Mol. Sci. 2024, 25, 2034. Ma, T.; Zhang, Y.; Li, Y.; Zhao, Y.; Attiogbe, K.B.; Fan, X.; Fan, W.; Sun, J.; Luo, Y.; Yu, X.; Ji, W.; Cheng, X.; Wu, X. The Resistance of Soybean Variety Heinong 84 to Apple Latent Spherical Virus Is Controlled by Two Genetic Loci. Int. J. Mol. Sci. 2024, 25, 2034.

Abstract

Apple latent spherical virus (ALSV) is widely used as a virus-induced gene silencing (VIGS) vector for function genome study. However, the application of ALSV in soybean is limited by the resistance of many varieties. In this study, the genetic loci linked to the resistance of a resistant soybean variety Heinong 84 was mapped by high-throughput sequencing‒Bulk Segregation Analysis (HTS‒BSA) using a hybrid population crossed from Heinong 84 and a susceptible variety Zhonghuang 13. Results showed that the resistance of Heinong 84 to ALSV is controlled by two genetic loci located on chromosome 2 and 11, respectively. Cleaved amplified polymorphic sequences (CAPS) markers were developed for identification and genotyping. Inheritance and biochemical analyses suggest that the resistance locus on chromosome 2 plays a dominant dose-dependent role, while the other locus contributes a secondary role in resisting ALSV. The resistance locus on chromosome 2 might encode a protein that can directly inhibit viral proliferation, while the secondary resistance locus on chromosome 11 may encode a host factor required for viral proliferation. Together, these data reveal novel insights on the resistance mechanism of Heinong 84 to ALSV, which will benefit for the application of ALSV as a VIGS vector.

Keywords

apple latent spherical virus; bulk segregation analysis; resistance locus; soybean; Heinong 84

Subject

Biology and Life Sciences, Virology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0
Get PDF Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.