Cuellar, P.; Castañeda-Ortiz, E.J.; Rosales-Zarza, C.; Martínez-Rodríguez, C.E.; Canela-Pérez, I.; Rodríguez, M.A.; Valdés, J.; Azuara-Liceaga, E. Genome-Wide Classification of Myb Domain-Containing Protein Families in Entamoeba invadens. Genes2024, 15, 201.
Cuellar, P.; Castañeda-Ortiz, E.J.; Rosales-Zarza, C.; Martínez-Rodríguez, C.E.; Canela-Pérez, I.; Rodríguez, M.A.; Valdés, J.; Azuara-Liceaga, E. Genome-Wide Classification of Myb Domain-Containing Protein Families in Entamoeba invadens. Genes 2024, 15, 201.
Cuellar, P.; Castañeda-Ortiz, E.J.; Rosales-Zarza, C.; Martínez-Rodríguez, C.E.; Canela-Pérez, I.; Rodríguez, M.A.; Valdés, J.; Azuara-Liceaga, E. Genome-Wide Classification of Myb Domain-Containing Protein Families in Entamoeba invadens. Genes2024, 15, 201.
Cuellar, P.; Castañeda-Ortiz, E.J.; Rosales-Zarza, C.; Martínez-Rodríguez, C.E.; Canela-Pérez, I.; Rodríguez, M.A.; Valdés, J.; Azuara-Liceaga, E. Genome-Wide Classification of Myb Domain-Containing Protein Families in Entamoeba invadens. Genes 2024, 15, 201.
Abstract
Entamoeba histolytica, the causative agent of amebiasis, is the third leading cause of death among parasitic diseases globally. Its life cycle includes encystation, which has been mostly studied in Entamoeba invadens, responsible of reptilian amebiasis. However, the molecular mechanisms underlying this process are not fully understood. Therefore, we focused on the identification and characterization of Myb proteins, that regulate the expression of encystation-related genes in various protozoan parasites. Through bioinformatic analysis, we identified 47 genes in E. invadens encoding MYB-domain-containing proteins. These were classified into single-repeat 1R (19), 2R-MYB proteins (27), and one 4R-MYB protein. The in silico analysis suggests that these proteins are multifunctional, participating in transcriptional regulation, chromatin remodeling, telomere maintenance, and splicing. Transcriptomic data analysis revealed expression signatures of eimyb genes, suggesting a potential orchestration in the regulation of early and late encystation-excystation genes. Furthermore, we identified 3135 probable target genes associated with reproduction, meiotic cell cycle, ubiquitin-dependent protein catabolism, and endosomal transport. In conclusion, our findings suggest that E. invadens Myb proteins regulate stage-specific proteins and a wide array of cellular processes. This study provides a foundation for further exploration of the molecular mechanisms governing encystation and unveils potential targets for therapeutic intervention in amebiasis.
Keywords
MYB-DBD containing proteins; encystation-excystation; transcriptional regulation; protozoan; Myb Recognition Element
Subject
Biology and Life Sciences, Parasitology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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