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Identification and Characterization of Nigrospora spp. and a Novel Species Nigrospora Wuhuensis Causing Black Leaf Spot on Rice and Wild-Rice in the Anhui Province of China
Liu, Y.; An, J.; Safdar, A.; Shen, Y.; Sun, Y.; Shu, W.; Tan, X.; Zhu, B.; Xiao, J.; Schirawski, J.; He, F.; Zhu, G. Identification and Characterization of Nigrospora Species and a Novel Species, Nigrospora anhuiensis, Causing Black Leaf Spot on Rice and Wild Rice in the Anhui Province of China. J. Fungi2024, 10, 156.
Liu, Y.; An, J.; Safdar, A.; Shen, Y.; Sun, Y.; Shu, W.; Tan, X.; Zhu, B.; Xiao, J.; Schirawski, J.; He, F.; Zhu, G. Identification and Characterization of Nigrospora Species and a Novel Species, Nigrospora anhuiensis, Causing Black Leaf Spot on Rice and Wild Rice in the Anhui Province of China. J. Fungi 2024, 10, 156.
Liu, Y.; An, J.; Safdar, A.; Shen, Y.; Sun, Y.; Shu, W.; Tan, X.; Zhu, B.; Xiao, J.; Schirawski, J.; He, F.; Zhu, G. Identification and Characterization of Nigrospora Species and a Novel Species, Nigrospora anhuiensis, Causing Black Leaf Spot on Rice and Wild Rice in the Anhui Province of China. J. Fungi2024, 10, 156.
Liu, Y.; An, J.; Safdar, A.; Shen, Y.; Sun, Y.; Shu, W.; Tan, X.; Zhu, B.; Xiao, J.; Schirawski, J.; He, F.; Zhu, G. Identification and Characterization of Nigrospora Species and a Novel Species, Nigrospora anhuiensis, Causing Black Leaf Spot on Rice and Wild Rice in the Anhui Province of China. J. Fungi 2024, 10, 156.
Abstract
Rice production in the Anhui province is threatened by fungal diseases. We obtained twenty-five fungal isolates from rice and wild-rice leaves showing leaf spot disease collected along the Yangtze River. A phylogenetic analysis based on internal transcribed spacer (ITS), translation elongation factor 1 alpha (TEF1-α), and beta tubulin (TUB2) sequences revealed that one isolate (SS-2-JB-1B) grouped with Nigrospora shaerica, one (QY) with Nigrospora chinensis, twenty-two with Ngrospora oryzae, and one strain (QY-2) grouped in its own clade, related to but clearly different from N. oryzae. Nineteen tested isolates including sixteen strains from the N. oryzae clade and the three isolates of the other three clades, caused disease on detached rice leaves. The three isolates that did not belong to N. oryzae were also able to cause disease on rice seedlings, suggesting that they were rice pathogens. Isolate QY-2 differed from the other isolates in terms of colony mor-phology, cell-size, and susceptibility to fungicides, indicating that this isolate represents a new species that we named Nigrospora anhuiensis. Our analysis showed that N. sphaerica, N. chinensis, and the new species N. anhuiensis can cause rice leaf spot disease in the field. This research pro-vides new knowledge for understanding rice leaf spot disease.
Biology and Life Sciences, Agricultural Science and Agronomy
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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