Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Advanced Platelet Lysate Aerogels: Biomaterials for Regenerative Applications

Fahd Tibourtine
,
Thibault Canceill
ORCID logo ,
Andrea Marfoglia
,
Philippe Lavalle
,
Laure Gibot
ORCID logo ,
Ludovic Pilloux
ORCID logo ,
Claire Medemblik
,
Dominique Goudouneche
,
Agnès Dupret-Bories
* ORCID logo ,
Sophie Cazalbou
*
Version 1 : Received: 29 December 2023 / Approved: 3 January 2024 / Online: 3 January 2024 (05:41:22 CET)

A peer-reviewed article of this Preprint also exists.

Tibourtine, F.; Canceill, T.; Marfoglia, A.; Lavalle, P.; Gibot, L.; Pilloux, L.; Aubry, C.; Medemblik, C.; Goudouneche, D.; Dupret-Bories, A.; Cazalbou, S. Advanced Platelet Lysate Aerogels: Biomaterials for Regenerative Applications. J. Funct. Biomater. 2024, 15, 49. Tibourtine, F.; Canceill, T.; Marfoglia, A.; Lavalle, P.; Gibot, L.; Pilloux, L.; Aubry, C.; Medemblik, C.; Goudouneche, D.; Dupret-Bories, A.; Cazalbou, S. Advanced Platelet Lysate Aerogels: Biomaterials for Regenerative Applications. J. Funct. Biomater. 2024, 15, 49.

Abstract

Human Platelet lysate, a hemoderivative product rich in growth factors, holds significant promise in tissue engineering and regenerative medicine. However, its practical utility in liquid or gel form is hampered by challenges such as limited stability and handling difficulties. This study aimed to engineer dry and porous aerogels from platelet lysate hydrogel using an environmen-tally friendly supercritical CO2-based shaping process customized for tissue engineering appli-cations. The resulting aerogels demonstrated remarkable mechanical robustness and improved manageability. Notably, they exhibited a high-water absorption capacity. Moreover, these aer-ogels exhibited a sustained and favourable biological response in-vitro. They demonstrated the ability to release functional growth factors, sustaining cellular metabolic activity similar to that of conventional culture conditions, even after prolonged storage. Furthermore, they supported the adhesion and proliferation of murine fibroblasts (BALB-3T3) and the migration of Human Um-bilical Vein Endothelial Cells (HUVEC). In addition to serving as excellent matrices for cell culture, these proposed aerogels also function as efficient growth factor delivery systems. This multi-functional capability positions them as promising candidates for various tissue regeneration strategies. Importantly, the elaborated aerogels can be conveniently stored and considered as ready-to-use products, enhancing their practicality and applicability in regenerative medicine

Keywords

Human Platelet lysate; supercritical carbon dioxide; Advanced Therapy Medicinal Products (ATMP); gels; regenerative medecine

Subject

Biology and Life Sciences, Biology and Biotechnology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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