Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Detection of hepatitis E virus (HEV) in pork sold in Saint-Louis, the North of Senegal

Sophie Deli Tene
,
Abou Abadallah Malick Diouara
* ORCID logo ,
Alé Kane
,
Sarbanding Sane
,
Seynabou Coundoul
,
Fatou Thiam
ORCID logo ,
Cheikh Momar Nguer
,
Mamadou DIOP
,
Mame Ndew MBAYE
,
Malick Mbengue
,
Seynabou Lo
,
Halimatou Diop Ndiaye
,
Coumba Toure Kane
,
Ahidjo Ayouba
ORCID logo
Version 1 : Received: 29 December 2023 / Approved: 29 December 2023 / Online: 29 December 2023 (14:30:10 CET)

A peer-reviewed article of this Preprint also exists.

Tene, S.D.; Diouara, A.A.M.; Kane, A.; Sané, S.; Coundoul, S.; Thiam, F.; Nguer, C.M.; Diop, M.; Mbaye, M.N.; Mbengue, M.; et al. Detection of Hepatitis E Virus (HEV) in Pork Sold in Saint-Louis, the North of Senegal. Life 2024, 14, 512, doi:10.3390/life14040512. Tene, S.D.; Diouara, A.A.M.; Kane, A.; Sané, S.; Coundoul, S.; Thiam, F.; Nguer, C.M.; Diop, M.; Mbaye, M.N.; Mbengue, M.; et al. Detection of Hepatitis E Virus (HEV) in Pork Sold in Saint-Louis, the North of Senegal. Life 2024, 14, 512, doi:10.3390/life14040512.

Abstract

The hepatitis E virus (HEV) is a zoonotic pathogen with a variety of hosts, including pigs, which act as reservoirs. In industrialized countries, sporadic cases caused by genotype 3, contracted by ingestion of contaminated uncooked or undercooked meat, have been reported. However, in developing countries, HEV infection is mainly dominated by genotype 2 and often associated with poor hygiene conditions and drinking water supplies. In West Africa, HEV infection and its circulation in domestic fauna are very poorly documented. The aim of this study was to assess the presence of HEV in pork sold in Saint-Louis. Meat products were collected between 20 and 25 August 2022 in three location of Saint-Louis region. After purchasing 250 g of sample, we minced 2 g of sample for extraction of total nucleic acids using the Purelink™ Viral DNA/RNA kit and then RT-PCR using the One-Taq™ One-Step RT-PCR kit targeting the HEV ORF2 genomic region. The products obtained were visualized on a 1% agarose gel. Of a total of 74 samples, divided into pork meat (n=65) and pork liver (n=9), 5.4% (n=4) were tested positive for HEV. In both cases, 2 samples were positive, representing a rate of 3.1% and 22.2% for meat and pork liver respectively. All new viral sequences obtained form a monophyletic group within HEV genotype 3. These preliminary results reveal a potential circulation of HEV in the pig population. The high proportion of contamination in the pork liver samples highlights a major risk associated with their consumption.

Keywords

hepatitis E virus; HEV-3; foodborne disease; domestic wildlife; zoonotic risk; Saint-Louis

Subject

Biology and Life Sciences, Virology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0
Get PDF Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.