Serrano, G.E.; Walker, J.; Nelson, C.; Glass, M.; Arce, R.; Intorcia, A.; Cline, M.P.; Nabaty, N.; Acuña, A.; Huppert Steed, A.; Sue, L.I.; Belden, C.; Choudhury, P.; Reiman, E.; Atri, A.; Beach, T.G. Correlation of Presynaptic and Postsynaptic Proteins with Pathology in Alzheimer’s Disease. Int. J. Mol. Sci.2024, 25, 3130.
Serrano, G.E.; Walker, J.; Nelson, C.; Glass, M.; Arce, R.; Intorcia, A.; Cline, M.P.; Nabaty, N.; Acuña, A.; Huppert Steed, A.; Sue, L.I.; Belden, C.; Choudhury, P.; Reiman, E.; Atri, A.; Beach, T.G. Correlation of Presynaptic and Postsynaptic Proteins with Pathology in Alzheimer’s Disease. Int. J. Mol. Sci. 2024, 25, 3130.
Serrano, G.E.; Walker, J.; Nelson, C.; Glass, M.; Arce, R.; Intorcia, A.; Cline, M.P.; Nabaty, N.; Acuña, A.; Huppert Steed, A.; Sue, L.I.; Belden, C.; Choudhury, P.; Reiman, E.; Atri, A.; Beach, T.G. Correlation of Presynaptic and Postsynaptic Proteins with Pathology in Alzheimer’s Disease. Int. J. Mol. Sci.2024, 25, 3130.
Serrano, G.E.; Walker, J.; Nelson, C.; Glass, M.; Arce, R.; Intorcia, A.; Cline, M.P.; Nabaty, N.; Acuña, A.; Huppert Steed, A.; Sue, L.I.; Belden, C.; Choudhury, P.; Reiman, E.; Atri, A.; Beach, T.G. Correlation of Presynaptic and Postsynaptic Proteins with Pathology in Alzheimer’s Disease. Int. J. Mol. Sci. 2024, 25, 3130.
Abstract
Synaptic transmission is essential for nervous system function and the loss of synapses is a known major contributor to dementia. Alzheimer’s disease dementia (ADD) is characterized by synaptic loss in the mesial temporal lobe and cerebral neocortex, brain areas associated with memory and cognition. The association of synaptic loss and ADD was established in the late 1980s and it has been estimated that 30-50% of neocortical synaptic protein is lost in ADD, but there has not yet been a quantitative profiling of different synaptic proteins in different brain regions in ADD from the same individuals. Very recently, PET imaging of synapses is being developed, accelerating the focus on the role of synaptic loss in ADD and other conditions. In this study, we quantified densities of two synaptic proteins, the presynaptic protein SNAP25 and the postsynaptic protein PSD95 in human brain using enzyme-linked immunosorbent assays (ELISA). Protein was extracted from the cingulate, hippocampus, frontal, visual, and entorhinal cortex from cognitively unimpaired controls, subjects with mild cognitive impairment (MCI) and demented subjects with different levels of Alzheimer’s pathology. SNAP25 is significantly reduced in ADD when compared to controls in frontal cortex, visual cortex and cingulate; while hippocampus showed a smaller, non-significant reduction and entorhinal cortex concentrations were not different. In contrast, all brain areas showed lower PSD95 concentrations in ADD when compared to non-demented controls, although in hippocampus this failed to reach significance. Interestingly, cognitively unimpaired cases with high levels of AD pathology had higher levels of both synaptic proteins in all brain regions. SNAP25 and PSD95 concentrations significantly correlated with densities of neurofibrillary tangles, amyloid plaques and Mini Mental State Examination scores. Our results suggest that synaptic transmission is affected by ADD in multiple brain regions. Differences were less marked in entorhinal cortex and hippocampus, most likely due to a ceiling effect imposed by the very early development of neurofibrillary tangles in older people in these brain regions.
Biology and Life Sciences, Neuroscience and Neurology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
,
,
,
,
,
,
,
