Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Molecular DNA Barcoding and Fertilization Effects on Growth and Herbal Quality Features of Cultivated Sideritis syriaca subsp. syriaca, a Local Endemic Plant of Crete with High Medicinal Value

Konstantinos A Paschalidis
* ,
Dimitrios Fanourakis
,
Georgios Tsaniklidis
ORCID logo ,
Ioannis Tsichlas
,
Vasileios A. Tzanakakis
,
Fotis Bilias
ORCID logo ,
Eftichia Samara
,
Ioannis Ipsilantis
ORCID logo ,
Katerina Grigoriadou
ORCID logo ,
Ioulietta Samartza
ORCID logo ,
Theodora Matsi
,
Georges Tsoktouridis
* ,
Nikos Krigas
Version 1 : Received: 14 December 2023 / Approved: 22 December 2023 / Online: 22 December 2023 (17:07:02 CET)

A peer-reviewed article of this Preprint also exists.

Paschalidis, K.; Fanourakis, D.; Tsaniklidis, G.; Tsichlas, I.; Tzanakakis, V.A.; Bilias, F.; Samara, E.; Ipsilantis, I.; Grigoriadou, K.; Samartza, I.; Matsi, T.; Tsoktouridis, G.; Krigas, N. DNA Barcoding and Fertilization Strategies in Sideritis syriaca subsp. syriaca, a Local Endemic Plant of Crete with High Medicinal Value. Int. J. Mol. Sci. 2024, 25, 1891. Paschalidis, K.; Fanourakis, D.; Tsaniklidis, G.; Tsichlas, I.; Tzanakakis, V.A.; Bilias, F.; Samara, E.; Ipsilantis, I.; Grigoriadou, K.; Samartza, I.; Matsi, T.; Tsoktouridis, G.; Krigas, N. DNA Barcoding and Fertilization Strategies in Sideritis syriaca subsp. syriaca, a Local Endemic Plant of Crete with High Medicinal Value. Int. J. Mol. Sci. 2024, 25, 1891.

Abstract

Herein, we applied DNA Barcoding for the genetic characterization of Sideritis syriaca subsp. syriaca (Lamiaceae; threatened local Cretan endemic plant), using seven molecular markers of cpDNA. Five fertilization schemes were evaluated comparatively in a pilot cultivation in Crete. Chemical fertilizers (ChF), integrated nutrient management fertilizers (INM) and two biostimulants were utilized (foliar and soil application). Plant growth, leaf chlorophyll fluorescence and colour were assessed, and leaf content of chlorophyll, key antioxidants (carotenoids, flavonoids, phenols), and minerals were evaluat-ed. Fertilization regime did not affect leaf colour but induced distinct differences in leaf shape altering quality characteristics. INM-foliar and ChF-soil application promoted yield, without affecting tissue water content or biomass partitioning to inflorescences. ChF-foliar application was the most stimulato-ry treatment for enhanced antioxidant compound content, while INM-biostimulant was the least effec-tive one. In conclusion, adverse fertilization options appear as optimal when considering yield or anti-oxidant compound content. New DNA sequence datasets for the plastid regions of petB/petD, rpoC1, psbK-psbI and atpF/atpH have been deposited in the GenBank for S. syriaca subsp. syriaca while the mo-lecular markers rbcL, trnL/trnF, psbA/trnH were compared to those of another 15 Sideritis species re-trieved from the GenBank, constructing a phylogenetic tree to show their genetic relatedness.

Keywords

Molecular markers, genetic characterization, antioxidant, carotenoids, flavonoids, Greece, phenols, bi-ostimulant; malotira; Mountain tea

Subject

Biology and Life Sciences, Agricultural Science and Agronomy

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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