Version 1
: Received: 19 December 2023 / Approved: 20 December 2023 / Online: 20 December 2023 (10:56:42 CET)
Version 2
: Received: 15 January 2024 / Approved: 16 January 2024 / Online: 16 January 2024 (09:58:41 CET)
How to cite:
Couto, M.R.; Rodrigues, J.L.; Dias, O.; Rodrigues, L.R. Saccharomyces cerevisiae as a Host for Chondroitin Production. Preprints2023, 2023121479. https://doi.org/10.20944/preprints202312.1479.v2
Couto, M.R.; Rodrigues, J.L.; Dias, O.; Rodrigues, L.R. Saccharomyces cerevisiae as a Host for Chondroitin Production. Preprints 2023, 2023121479. https://doi.org/10.20944/preprints202312.1479.v2
Couto, M.R.; Rodrigues, J.L.; Dias, O.; Rodrigues, L.R. Saccharomyces cerevisiae as a Host for Chondroitin Production. Preprints2023, 2023121479. https://doi.org/10.20944/preprints202312.1479.v2
APA Style
Couto, M.R., Rodrigues, J.L., Dias, O., & Rodrigues, L.R. (2024). <em>Saccharomyces cerevisiae </em>as a Host for Chondroitin Production. Preprints. https://doi.org/10.20944/preprints202312.1479.v2
Chicago/Turabian Style
Couto, M.R., Oscar Dias and Lígia R Rodrigues. 2024 "<em>Saccharomyces cerevisiae </em>as a Host for Chondroitin Production" Preprints. https://doi.org/10.20944/preprints202312.1479.v2
Abstract
Chondroitin is a glycosaminoglycan that has gained widespread use in nutraceuticals and pharmaceuticals, mainly for treating osteoarthritis. Traditionally, it has been extracted from animal cartilage but recently, biotechnological processes have emerged as a commercial alternative to avoid the risk of viral or prion contamination and offer a vegan-friendly source. Typically, these methods involve producing the chondroitin backbone using pathogenic bacteria and then modifying it enzymatically through the action of sulfotransferases. Despite the challenges of expressing active sulfotransferases in bacteria, the use of eukaryotic microorganisms is still limited to a few works using Pichia pastoris. To create a safer and efficient biotechnological platform, we have constructed a biosynthetic pathway for chondroitin production in S. cerevisiae as a proof-of-concept. Up to 125 mg/L and 200 mg/L of intracellular and extracellular chondroitin were produced, respectively. Furthermore, as genome-scale models are valuable tools for identifying novel targets for metabolic engineering, a stoichiometric model of chondroitin-producing S. cerevisiae has been developed and used in optimization algorithms. Our research has yielded several novel targets, such as uridine diphosphate(UDP)-N-acetylglucosamine pyrophosphorylase (QRI1), glucosamine-6-phosphate acetyltransferase (GNA1) or N-acetylglucosamine-phosphate mutase (PCM1) overexpression that might enhance chondroitin production, which will guide future experimental research to develop more efficient host organisms for the biotechnological production process.
Biology and Life Sciences, Biology and Biotechnology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Received:
16 January 2024
Commenter:
Joana Rodrigues
Commenter's Conflict of Interests:
Author
Comment:
The manuscript was modified before being submited to other journal (IJMS). Modifications: -Title - order os the sections - In vivo production was moved to the beggining of the paper and in silico material to the end of the paper - slight modifications were made to some paragraphs (e.g., abstract, conclusions) to accommodate the previous modifications
Commenter: Joana Rodrigues
Commenter's Conflict of Interests: Author
Modifications:
-Title
- order os the sections - In vivo production was moved to the beggining of the paper and in silico material to the end of the paper
- slight modifications were made to some paragraphs (e.g., abstract, conclusions) to accommodate the previous modifications