A Comprehensive Review on Deep Eutectic Solvents and Its Use to Extract Bioactive Compounds of Pharmaceutical Interest

1. Introduction

Everything around us is chemistry! From the atoms that make us up, to the food we eat and the clothes we wear. Chemistry extends to all industries, whether in the pharmaceutical, textile, cosmetic, food or agricultural areas. Thus, chemistry is one of the areas of science responsible for the development of society and the quality of life as we know today. However, when misused, chemistry also has consequences, namely at the environmental level and for human health. Chemists are primarily responsible for the use and development of new materials and, consequently, to produce hazardous substances for the environment and humans. Thus, chemists have an increased responsibility in achieving sustainable development [1,2,3,4,5,6].

In the nineties of the last century, with a view to combating all these problems, a new branch of chemistry emerged: Green Chemistry. IUPAC (International Union of Pure and Applied Chemistry) defines green chemistry as “the invention, development and application of chemical products and processes to reduce or eliminate the use and formation of substances dangerous” [4]. This concept is based on 12 principles, which were introduced in 1998 by Paul Anastas and John Warner [7].

With the 12 principles of green chemistry in mind, industry in general, and the pharmaceutical in particular, must change the way they produce to aim to a more sustainable product. One of the main sources of pollution of the pharmaceutical, and chemical industries are the solvents that are used. Solvents are chemical substances with variable constitution from natural or synthetic sources, which are commonly used to dissolve, dilute, or disperse other compounds [8]. Solvents determine the solubility of their solutes, which is a property on which several processes are based, namely, extractions, separations, purifications, and concentration of substances. Solvents, therefore, play a crucial role in each of the processes, are used abundantly [9].

Solvents are fundamental elements in extractions of bioactive substances from natural products, whether as dissolution reagents or simply to maintain proper molecular interactions in transformations [9]. Among the numerous solvents that can be used as extraction solvents for natural bioactive compounds, n-Hexane, petroleum ether, diethyl ether, ethyl acetate, chloroform dichloromethane, acetone, n-butanol, ethanol, methanol, and water are the most used ones. Polar solvents such as water, ethanol and methanol are commonly used as extraction solvents for polar compounds, while non-polar solvents such as hexane and petroleum ether are used as extraction solvents for non-polar compounds. The remaining solvents allow the extraction of bioactive compounds of intermediate polarity [10]. However, except for water, these solvents are harmful for the environment and to community health [11].

But what would be an ideal solvent?

The ideal solvent would be a fully sustainable compound, an innocuous substance without any type of negative impact, both for the environment and for humans, that simultaneously favoured the process conditions, making them less complex. To obtain it, a renewable raw material would be necessary and the process of obtaining it would not generate waste or emit pollutants. Subsequently, its preparation process would have to be efficient both in economic terms and in energy terms. The application of the ideal solvent would be versatile and would contribute to the superior performance of the process. In this way, the use of an ideal solvent in a chemical process would allow reducing the amount used allowing an easy elimination through recycling. The ideal solvent should be completely biodegradable, as if it had never existed. Unfortunately, we are still far from using ideal solvents [12,13,14,15,16].

Water is undoubtedly the solvent that comes closest to the ideal solvent. It is nontoxic, non-inflammable low-cost substance. But water has also some disadvantages, do not dissolve polar compounds due to the high dielectric constant (at room temperature and normal pressure), has a hight boiling point which entails high energetic cost for its removal. Additionally, the scarcity of water in some regions of the world and the possible contamination of the effluents due to difficulties during the separation processes are also problems to the use of water as a solvent [14,17,18,19,20,21].

The simplest solution to the solvent use problem would be to develop methods that do not require solvents, such as mechanochemical methods [22,23,24], however, this is not always feasible and therefore these compounds should be avoided or replaced by innocuous solvents for the same purposes [1,12,17,25,26,27,28].

2. Deep Eutectic Solvents

Neoteric solvents emerged as an alternative to organic solvents and are commonly called green solvents, since they have low toxicity, are biodegradable, are made with accessible and low cost materials, and are quite easy to make [12,13,14,15,16]. This class solvents follow some of the 12 principles of green chemistry mentioned before, as they come from a natural origin have low toxicity, are biodegradable, and minimize waste as much as possible. Among them are included supercritical fluids, thermomorphic solvents, switchable solvents, liquid polymers, solvents derived from biomass, fluorinated solvents, ionic liquids (ILs) and deep eutectic solvents (DESs) [16,17,20,21,28,29].

In this review paper we will focus on DESs, however several reviews can be found on neoteric solvents and their applications [13,14,15,20,21,29,30].

The first Deep Eutectic Solvent (DES) was a mixture of choline chloride (Tºm=302ºC) and urea (Tºm=133ºC) in a 1:2 ratio, which led to the formation of a transparent liquid with a melting point of 12°C, much lower than that of its individual constituents, and which exhibited quite different properties [31,32,33,34,35].

The concept of eutectic mixture is based on the word Eutectic, originating from the ancient Greek word eutēktos, which means “easily melted” and is interpreted as the decrease in the melting point after the combination of its constituents [30,36]. A eutectic mixture is defined as a mixture of two or more components that results in a significant decrease in the melting point compared to the initial pure compounds [16]. The eutectic system can be represented through a phase diagram of temperature as a function of the composition of the eutectic mixture (Figure 1(a)). As the two compounds, A and B, mix, their respective melting temperatures (T°m (A) and T°m (B)) decrease until they reach a minimum point, called the eutectic temperature of the mixture. The eutectic point represents the composition and minimum melting temperature at which the melting curves of these two constituents intersect. A DES (Figure 1(b)) is a non-ideal eutectic mixture in which an abrupt depression of the eutectic point occurs, hence the term deep [30,36,37].

DESs are defined as a mixture of two or more pure compounds, which, when combined in an appropriate ratio, give rise to a eutectic mixture that deviate from the ideal thermodynamic behavior. This deviation is due to strong interactions between the initial components that act as hydrogen bond donors (HBDs) and hydrogen bonds acceptors (HBAs). The HBDs and HBAs interact in the DESs to form a dense network of molecules that give them remarkably interesting physical and chemical properties. These properties include a low melting point, low volatility, high thermal stability, and a high solubilizing power of a wide range of compounds, namely those poorly soluble in water [16,17,21,26,30,31,36,38,39,40,41,42]. DESs are also customizable solvents, meaning that their physical and chemical properties can be adjusted by changing their constituents [36,41]. Consequently, it is very easily to obtain a large variety of DESs with different properties and applications, always with a maximum efficiency [30,32,33,41,42].

The most used HBA is choline chloride due to its low cost and biodegradability [42]. Choline chloride is the most economical synthetic way to obtain choline and can be produced on a large scale, it can be extracted from biomass, or it can be synthesized from fossil reserves [34]. However, other compounds are also used as HBAs, namely betaine, alanine, glycine, lidocaine, among others. Regarding HBDs, compounds such as thiourea, urea, citric acid, malic acid, tartaric acid, glycerol, menthol, xylitol, and oxalic acid are often used (Figure 2) [16,34,36,39,41,43,44,45,46]. Water can also be present in its composition and can play various roles, such as HBA, hydrogen bond donor (HBD), or simply as a dilution solvent [36].

DESs have several advantages when compared with other neoteric solvents, as for example ILs. DESs are extremely easy to prepare with a high purity and a 100 % atom economy and without forming any by-product [16,47]. DESs do not require any purification step or waste disposal, have a low toxicity, reducing adverse environmental and human effects [16].

The main disadvantages of DESs are their high density and viscosity [40,41] which reduce their fluidity, making it difficult to transfer solvents and masses, especially in continuous operations, and in dissolutions [9]. This obstacle can be overcome by applying external physical forces such as microwaves and high temperatures or addition of water [40,41]. Furthermore, low vapor pressures contribute to the low volatility of DESs, which makes it difficult to separate them when needed. This especially important in their use as extraction solvents.

The applications and potential applications of deep eutectic solvents are immense, highlighting their use as solvents [38], extraction solvents [31,44,48,49,50,51,52,53,54], electrolytes for lithium batteries [55], in nanotechnology [56], gas capture [57], for the synthesis of new materials [58,59,60], in chromatography [44,61], for electrochemical analysis [62], in biomedical applications [63], for removing excess glycerol in biodiesel [64]. More recently, deep eutectic solvents have been applied in the pharmaceutical industry with the aim of improving the bioavailability of drugs [65]. This is achieved by increasing the active pharmaceutical ingredient (API) solubility [66,67,68], permeability [69,70], stability [71,72], as well as a controlled release of drugs [73,74].

In this review we will focus on the applications of DESs for the extraction of bioactive compounds with pharmacological interest.

2.1. Deep Eutectic Solvents Classification

DESs can be classified as hydrophobic or hydrophilic according to their solubility in water [16]. The majority of the DESs are hydrophilic due to the extensive network of hydrogen bonds [30,75]. Hydrophobic DESs are defined as insoluble or very poorly soluble substances in water, composed of two or more compounds insoluble in water [30,76]. Hydrophobic DESs have been successfully applied in several areas, namely for water purification [77], in the preparation of new materials such as magnetic gels, in nanoparticles consisting of carbon nanotubes and graphene for the removal of organic micropollutants and metallic ions from water [78,79], in the capture of carbon dioxide [80], in electrolyte medium for solar cells [81] and for the extraction of bioactive compounds [82,83].

DESs are usually classified according to the type of compounds used in their preparation and are subdivided into four subclasses: Natural deep eutectic solvents (NADESs), Therapeutic deep eutectic solvents (THEDESs), Polymeric deep eutectic solvents (PDESs) and Poly-quasi eutectic solvents (PQDESs) [30].

NADES was discovered in 2011 when trying to elucidate the solubility of intracellular compounds, which were insoluble in water and lipids [30]. NADESs contain in their composition cellular metabolites such as amino acids, alcohols, sugars, and organic acids [40,42,84]. In addition, water can also be part of its composition, forming a ternary system [36]. In nature we can easily find this type DESs in different cells and organisms. For example, nectar is nothing more than a mixture of sugars that are solid at room temperature when separated, but liquid when combined. Another example is honey, with such interesting and unique properties that are not yet fully understood, but with tested medical applications [40,85,86,87]. NADESs play a key role in cellular metabolism and in many biological processes such as resistance to drought, germination, and dehydration. In addition, all living organisms have a process called organ cryopreservation, which is a defence mechanism to withstand extreme conditions, such as temperature variations between winter and summer. NADESs act as cryoprotective agents for the simple fact that membranes, enzymes, and metabolites remain stable with the addition of this type of eutectic mixtures [30,42]. In terms of applications, NADESs have been used in biocatalysts processes [35,88], in the extraction of compounds [89,90], in pre-treatment of biomass [91,92], in electrochemistry for detection of bioactive materials [93], for drug solubilization [94], permeation enhancement [95], and as extraction solvents [26,43].

THEDESs, emerged as one of the strategies to promote increased solubility, permeability and, consequently bioavailability of drugs [96,97]. THEDESs are a class of DESs that use at least one active pharmaceutical ingredient (API) as one of its components [30,73,98,99,100]. These solvents have raised a lot of interest and THEDESs are currently being studied, namely for increasing the solubility of drugs in aqueous solutions or increasing their permeability in different biological barriers such as the skin or intestinal wall, among others [66,95,99,101].

Another class of DESs are polymeric, so named because a portion of DES is polymerizable [30,102,103]. The polymer completely converted can be used in various applications, namely in nanotechnology [104] electrochromatography [105] and gas capture [102]. In 2017, a new class of DESs was proposed: the quasi-polymeric deep eutectic solvents [102].

2.2. Deep Eutectic Solvents Synthesis

DESs can be synthesized in varied ways depending on the equipment available (Figure 3). Independent on the type of equipment that is used, the synthesis involves the mixture of the two (or more) components normally without the need of any solvent, and then provide energy to the system during a certain amount of time in the form of a temperature increase (heating and stirring), irradiation (microwave and ultrasound), mechanical forces (grinding) or a combination between temperature and mechanical forces (twin screw extrusion). There also methods in which the initial components are dissolved in a solvent (normally water) and then heated in vacuum to evaporate the solvent (vacuum evaporation), or frozen and lyophilized (lyophilization) [30,41]. Another method that seems interesting in terms of sustainability is the use of concentrated solar radiation [106].

The temperature is needed it should be carefully chosen due to the possibility of degradation of the initial compounds [30,36,107,108,109,110].

The time needed to the DESs be synthesized may vary from minutes to hours and depending on the method of preparation and on the initial components and their ratio.

2.3. Deep Eutectic Solvents Properties

Deep eutectic solvents have a set of properties that make them quite useful as extraction solvents.

One of the features of DESs is the possibility of being used as extraction solvents for a wide range of solutes [13]. The main characteristic that makes them good extraction solvents is their solvation capacity, that is, the fact that they can accept and transfer protons and electrons, establishing hydrogen bonds with the compounds and retrieve them from their matrix [111]. DESs, are known for their enormous capacity to dissolve very poorly soluble metabolites in water. They are also able to dissolve natural products such as rutin, paclitaxel, gingilido b and quercetin, starch, deoxyribonucleic acid (DNA) and high molecular weight proteins [43]. Dai et al. [112] verified that, small molecules, such as rutin, paclitaxel, gingilido b increased the solubility values in DESs when compared with water. It was found that, for example, rutin is 50-100 times more soluble in DESs than in water. DESs are also capable of stabilizing natural products [42]. Natural pigments such as carthamine are more stable to light, elevated temperatures, and storage time in various DESs with sugars than in water or a 40% ethanol solution [113]. The same stabilizing effect was later observed in anthocyanins [114].

Polarity expresses the strength of a solvent, that is, it determines its solvation power and is an important characteristic for a solvent [36,42,115]. The polarity of a DES can be adjusted by changing its constituents, making it more polar or apolar accordingly to the necessities, improving the selectivity of the solvent towards a particular bioactive component or class of components. A relative polarity scale could be established, but there are few publications about the polarity of DESs. The most used scales are the Dimroth and Reichardt, however, these scales are not universal and depend on probes. This means that the polarity parameters obtained by different probes cannot be compared [30,36,116]. Variations in the polarity of DESs depend on the compositions of their individual constituents and are believed to be related to the molecular structure of the HBD [115,116]. As a rule, polarity increases with increasing intermolecular attractions. Omar et al. [116] found that for the same DES choline chloride/glycerol in different molar ratios 1:1, 1:2 and 1:3, the polarity values were of 58.49 kcal/mol, 58.00 kcal/mol and 57.96 kcal/mol, respectively.

DESs thermal stability is an important property because limits the maximum operating temperature for which DESs can be useful. Between the temperature of glass transition (Tg) and the decomposition temperature, the DESs maintains its liquid state and the properties that arise from that condition [117]. Delgado-Mellado et al. [117] studied the thermal stability of eight different choline chloride-based DESs and found out that the volatility of the HBDs was the main contributor to the decomposition of the DESs. The authors also emphasized the importance of establishing the real range of operational temperatures for DESs to be able to use them at industrial level. All DESs are normally glass formers with a Tg bellow 0ºC, however this property can be modified with the inclusion of water in the DESs structure due to the plasticizing effect of water. Craveiro et al showed that an increase in 5 wt% of water in a chlorine chloride:xylitol (2:1) DES, decreased the T_g in 4ºC [118]. The presence of water (added or absorbed during preparation) can also influence the thermal stability of the DESs if the water is lost upon heating. This can be observed in thermogravimetric analysis with mass lost around 100ºC due to water evaporation [119].

The toxicity of any compound depends on its ability to cross or interact with biological membranes and is affected if its structure is disrupted [120]. DESs are considered green solvents, presenting a low toxicity, which comes from the use of initial constituents of natural or little toxic origin. However, studies of the toxicity, cytotoxicity and ecotoxicity of DESs and respective aqueous mixtures are still too rare for them to be classified as safe [42,121,122]. Initial studies showed that DESs were biodegradable and non-toxic [123]. However, some DESs proved to be more toxic than their initial constituents [124].

Toxicity and cytoxicity studies made with DESs composed by different HBAs and HBDs in Gram-positive and Gram-negative bacteria and shrimp larva gave diverse results depending on the DESs. Some DESs showed to be toxic for some of the bacteria used and its toxicity was associated with the pH and with the charge delocalization between the HBA and HBD. The DESs also showed higher cytoxicity when compared to the initial components. The authors concluded that the lack of oxygen and their high viscosity may be the reason for this behaviour [124]. Fish cells and a human cell line were used to study the toxicity of three choline chloride DESs. One of the DESs showed a moderate toxicity due to the formation of calcium ions in addition with a pH decrease when the DES was added to the culture medium [123]. Lapena et al. [120] studied and evaluated the ecotoxicity of six DESs on algae, bacteria, and crustaceans. The authors concluded that the inclusion of water in the DESs can change the DES toxicity, because water it can be a part of the DESs or disrupt the intermolecular forces between the DESs components. Juneidi et al. [125] evaluated the toxicological profile of ten DESs on fungi and establish that the toxicity of the acidic DESs was higher since it is known that acid compounds can cause cell membrane and protein damage. Nevertheless, the DESs showed a lower toxicity than the respective acids when used isolated. The authors consider that this decrease can be explained by a pH change during the formation of the DESs or by a synergetic effect between the two initial compounds. The acidity of DESs was also considered a problem on a study made by Passos et al. [126] which evaluated the toxicological profile of nine DESs on an enzyme. All the DESs were constituted by sugars, organic acids, and water. The variation of the sugars was found to have no relation with the toxicity of the DESs, however the acidity of the organic acids was linked to have a direct relation with the increase on the DESs toxicity. Zhao et al. [127] studied twenty DESs that contained amines, alcohols, sugars, and organic acids as HBDs. Toxicity was evaluated for Gram-positive and Gram-negative bacteria. All DESs containing amines, alcohols, and sugars as HBDs showed no inhibition of any of the bacteria. Only the DESs constituted by the organic acids as HBDs significantly inhibited all bacteria (seven DESs out of twenty). Higher inhibition was found for Gram-negative bacteria. The authors concluded that the characteristic acidity of these compounds must be responsible for the damage caused to their outer membrane. This is extremely important, since Gram-negative bacteria have their own external membrane, which acts as a protective barrier, making them more resistant to external aggressions, therefore, these DESs are a hypothesis to combat this type of very resistant bacteria. Li et al [128] proposed a rating scale for DES toxicity: Type 1, Type 2, and Type 3. Type 1 is the DES that has a higher toxicity then the individual constituents due to new interactions created during the formation of DES. Type 2 is the DES that has lower toxicity then the initial constituents. In this case the properties that make the initial components toxic are modified in the DESs. Finally, Type 3 is the DESs whose toxicity is the combination of the toxicity of its constituents. The authors studied DESs with amino acids in their constitution and observed, for the first time, that DESs containing amino acids can also present toxicity.

There are few studies that evaluate the biodegradability of DESs. All DESs biodegradability studies follow the Standard OECD No.301 D which allows the classification of a compound as easily biodegradable or not in an aqueous aerobic medium. According to this standard, to consider a material easily biodegradable it is necessary that the level of biodegradation is 60% on the 10^th day out of 28 for respirometry methods [129]. Lapena et al. [120] studied the biodegradability of six DESs and concluded that the addition of water to the DESs affects their biodegradability, increasing or decreasing it depending on the DES. The number of hydroxyl groups was also a factor in the percentage of biodegradability of the DESs. The same conclusion was retrieved by Radošević et al. [123] that evaluated the aerobic biodegradability of three DESs with components containing different number of hydroxyl groups. The authors concluded that the higher the amount of hydroxyl groups the higher the percentage of biodegradability.

The density of the DESs is an extremely important property due to the implication it has on their use and handling. Very high densities can cause the DESs not to flow, which can impair their processing. The density values of DESs are higher than those of their pure constituents, and as a rule, DESs have values higher than those of water, except for hydrophobic DESs [36,39,115,116,130]. DESs are usually highly viscous solvents, which can impede mass transfer and decrease the extraction efficiency [111]. Viscosity translates resistance to deformation at a given shear rate of a given fluid [16,109,131,132]. A liquid with low viscosities flows very easily, while more viscous one’s flow more slowly This is particularly important, as it will influence and determine its commercial applicability and the cost of the process [39,42,131]. The high density and viscosity of DESs can be circumvent by adding water to the DESs and/or to handle them at temperatures higher than the ambient temperature.

One of the characteristics of the DESs is their low vapor pressure. This intrinsic characteristic of these compounds can be an advantage or a disadvantage depending on the application. In an extraction process it is preferable that the DESs have a lower vapor pressure, considering that the extraction temperature is reached without loss of extraction solvents by evaporation. However, if we intend to separate the DES from the extract à posteriori, a low vapor pressure is a disadvantage because it hinders its evaporation, unless that the DESs can be incorporated in the extracts or other methods are used to separate the DESs from the extracts [9,46,133].

3. Bioactive Compounds

Bioactive compounds are secondary metabolites that are present in plants, fungi, microorganisms, and animals and can cause pharmacological or toxicological effects in humans and animals [49,134,135]. Phytochemicals are bioactive compounds present in plants, such as fruits, vegetables, and cereals [136]. These compounds are classified into phenolic compounds (polyphenols), terpenoids and nitrogen-containing compounds. Phenolic compounds are the largest group of phytochemicals and are present in almost all plants in the form of secondary metabolites, where they play a key role both in growth and reproduction processes, as well as in protection against pathogens and predators [46,137,138]. Phenolic compounds can in turn be divided into flavonoids (anthocyanins, flavonols, flavones, isoflavones and flavols) and non-flavonoids (phenolic acids, stilbenes, lignin’s, and tannins), [139].

Bioactive compounds have been extensively investigated and their application as therapeutic agents has increased due to the important biological properties they possess, namely anti-inflammatory, antidiabetic, analgesic, anticancer, antimicrobial (antifungal and antiviral) and antioxidant activities, which makes them useful for the prevention of various diseases such as cardiovascular diseases [140], neurodegenerative diseases [97], and cancer [141]. Some of these compounds have also been shown to have anti-aging properties [142] and the ability to absorb ultraviolet radiation through the presence of chromophores in their composition, causing the entry of radiation into the skin to be blocked by these compounds, protecting it [143]. Hair growth promoting [144] and nail damage prevention [145] properties have also been reported. These characteristics make them extremely interesting compounds for applications in the pharmaceutical industry [135,137] (Figure 4).

3.1. Extraction of Bioactive Compounds

To obtain bioactive compounds, it is necessary to extract them from natural sources (Figure 5). The objective is to obtain sustainable compounds, so the entire process involving their extraction must comply with this requirement. This implies a raw material of natural and environmentally friendly origin, an environmentally friendly solvent and extraction method, and the lowest possible energy expenditure with the lowest possible waste production [146,147,148].

3.1.1. Extraction Techniques

The extraction of compounds with a bioactive potential is the first step in the research and development of new natural products. These extraction methods must be selective, economical, reproducible, environmentally friendly, safe, and effective [149]. Natural bioactive compound extraction techniques are divided into conventional and modern techniques (Figure 6).

Maceration, percolation, decoction, reflux and Soxhlet are some examples of conventional extraction methods and use solvents such as water or organic solvents such as methanol, ethanol, propanol, acetone, and ethyl acetate [149,150]. The non-selectivity of these conventional methods, the possibility of degradation or isomerization of the bioactive compounds due to prolonged heating, their low extraction yield, the negative environmental effects, their long extraction times, the high extraction costs associated with large amounts of organic solvents and energy costs, and the wrong methodologies for recycling the hazardous solvents that are used, have made it necessary to develop other more environmentally friendly techniques [135,149,150].

The so-called modern techniques were developed with the intention to reduce the use of toxic solvents, reducing the waste and energy consumption and at the same time increasing the extraction yield [115]. New methods were then proposed, such as enzyme-assisted extraction (EAE), fermentation-assisted extraction (FAE), mechanochemically assisted extraction (MCAE), ultrasound-assisted extraction (UAE), microwave-assisted extraction (MCAE), supercritical fluid extraction (SFE), and subcritical water extraction (SWE).

Enzyme-assisted extraction (EAE) is a biological extraction method that is based on the ability of enzymes to hydrolyse plant compounds, breaking their cell wall to release the cytoplasmic content, namely bioactive compounds, in an extraction solvent. Regarding its advantages, this extraction is a specific technology, given that the enzymes used are specific, such as cellulase, α-amylase, hemicellulose, pectinase, among many others [149,151,152,153]. Most of these enzymes are derived from microbial organisms or other various sources, such as plants and animals [115]. However, and this being its biggest disadvantage, some enzymes are expensive, especially on a large scale or on an industrial scale if there is a large substrate enzyme ratio, making this method expensive [153].

In fermentation-assisted extraction (FAE) microorganisms are used for fermentation of substrates in plant materials to facilitate the selective extraction and separation of target biomolecules from their complex matrices. Only microorganisms are used that already exist in the material itself, so it has a low associated cost. However, this technique is not specific, and sometimes there is formation of by-products or hydrolysis of the target compounds [149].

The extraction of pulsed electric field (PEF) is based on the application of intermittent pulses of high voltage current during short periods of time, in the order of micro to milliseconds, in a previously placed inside a chamber, located between two electrodes. This voltage generates an electric field, and its intensity is dependent both on the distance between the electrodes and on the applied voltage. When electric field exceeds the critical value, an electroporation phenomenon occurs. This phenomenon consists of the increased permeability of the cytoplasmic membrane to the passage of ions and macromolecules due to the repulsion between charged molecules. Plant materials are destroyed by disruption of their membrane and there is an increase in mass transfer during extraction, reducing extraction time. The main advantage of this method is the non-use of heating, which minimizes the degradation of thermolabile compounds and requires less energy. Moreover, the addition of chemicals is not necessary, which reduces the associated cost without affecting the quality of the product. This technique has some disadvantages such as an exceedingly high initial cost and a dependence on the conductivity of the environment [152].

Currently, mechanochemical methods have attracted a lot of attention due to the much higher extraction yields they present, their low cost, short extraction time, mild experimental conditions, simplicity of equipment and ease of coupling a posteriori with various methods of analysis. Mechanochemically assisted extraction (MCAE) is an extraction method that consists of applying a mechanical force (high-speed grinding) to the tissues and cell membranes of plant materials. This force facilitates the release of intracellular molecules and consequently the extraction of target compounds from their matrix. A solvent can be used in extremely low amounts to react with the target compounds under mechanical force improving the extraction yield. The purpose of this technique is not simply to increase the contact area between the target compounds and the solvent, but also to chemically transform them into forms that are more soluble in water or in the solvent [149,154]. The main advantages of this method include the simplicity of the extraction process, the use of a lower extraction temperature that allows the extraction of thermolabile compounds and reduces the cost of the process, a shorter extraction time, the use of low amount of solvents, if needed, and an increase in the selectivity of the extraction [155,156]. This technique proves to be far superior in the fragmentation of cells and tissues, homogenization of materials. However, the method has disadvantages, namely the difficulty of extracting compounds that are hydrophobic and neutral [149].

Ultrasound-assisted extraction (UAE) is one of the most used modern extraction techniques and is a technique already used to extract bioactive compounds since 1980. This method is based on the use of low frequency energy (20-1000 kHz) and high power (80-200 W), ultrasonic waves [115,152,157]. Upon application and penetration of ultrasonic waves into the plant matrix, cycles of compression and expansion occur in the medium that result in the phenomenon of cavitation and consequently the formation of air bubbles. The size of these bubbles varies during the expansion and compression cycle until it reaches its critical size and collapses with consequent release of energy. This causes the rupture of the cell wall of the plant material and the release of the bioactive compounds that are inside the cells [84,150,158]. As advantages, this method is quite simple since it only needs an ultrasonic bath, or an ultrasonic probe and it is not necessary a dispersive solvent to increase the surface area of contact between the extraction solvent and the sample [115,157,159]. In addition, it uses low amounts of solvent, consumes little energy and the extraction times are short. The phenomenon of cavitation improves the efficiency of the extraction, as it not only accelerates the dissolution and diffusion of bioactive compounds, but also allows heat transfer. Thus, significantly higher extraction yields are obtained than with other techniques. As disadvantages, this method is not applicable to thermolabile compounds and the noise from ultrasonic waves is annoying for the operator [151].

Microwave-assisted extraction (MAE) consists of using microwaves with frequencies in the 0.3–300 GHz range, which interact directly with the sample through dielectric heating. In this process, microwave electromagnetic radiation heats a dielectric material through a dipole rotation of the polar components present in the matrix. In this way, it leads to the degradation of plant cell tissues and induces the flow of ions, releasing the active compounds from the intracellular and cellular membrane [84,115,150]. As advantages, it is an easy-to-handle and efficient technique, with high extraction yields, short extraction times, low energy expenditure and lower consumption of solvents. Contrary to some techniques, it can analyse several samples simultaneously, which leads to a low extraction time [84,149]. Its major limitations are the fact that it is not applicable to thermolabile compounds or production at an industrial level due to the associated cost and to the impurities of the extracts obtained due to the intense extraction conditions that make it possible to extract different analytes, even the undesirable ones [149,150].

When a gas undergoes compression and heating, its physical properties as a gas change and it becomes a supercritical fluid. This fluid has the solvation power of a liquid and the diffusivity of a gas at temperature and pressure below the critical point [152,153]. The supercritical extraction technique is based on the introduction of a supercritical fluid into a plant material that will extract bioactive compounds according to their solubility. Co-solvents such as water, ethanol and methanol can be added to increase the solvation power of the supercritical fluid, which may allow the extraction of polar compounds [152,153]. Supercritical carbon dioxide is the most used. It allows the extraction of lipophilic compounds and fats, without the need for a concentration step [157]. In terms of advantages, the method uses green solvents without the need to use organic solvents which makes it environmentally friendly, the process can be automated, it is selective, it prevents sample oxidation, the extracted compounds are very stable, and it uses low temperatures. Furthermore, the low viscosities and high diffusivities of these fluids increase mass transfer, reducing extraction time. As disadvantages, the technique has a high associated cost, the equipment used is complex, consumes a high amount of energy and has low selectivity for polar compounds [152,153].

Subcritical water extraction (SWE) consists of extracting less polar compounds using water as the extraction solvent. Subcritical water is kept in liquid state under high pressure (10-60 bar) and temperature (100-374°C). At 25°C, the dielectric constant of water is 80. If the temperature is increased to, for example, 250°C and the pressure to 25 bar, the dielectric constant drops dramatically to 25, lying between that of methanol and ethanol, at 25°C. In this way, at these temperatures and pressures, water can extract compounds of medium to low polarity [152,160,161,162]. The advantages of this extraction method are its simplicity and the fact that it only uses water as a solvent, which makes the extraction technique green and with a lower associated cost. Polar, moderately polar, and non-polar compounds can be extracted separately using this technique, which is a great advantage, for example, compared to supercritical extraction. In addition, the technique has a short extraction time, high efficiency and enables a continuous process. However, it has disadvantages, namely the difficulty in separating the bioactive compounds from the extracts, the thermal degradation and oxidative damage that can occur when using high temperatures, the difficulty in cleaning the equipment [152,162].

4. Deep Eutectic Solvents for the Extraction of Bioactive Compounds

The use of DESs for bioactive extraction gained a lot of interest by the scientific community in the last 10 years. A rapid search in the WebofKnowledge platform with the keywords “Deep Eutectic Solvents Bioactive Compounds Extraction,” shows a rapid increase in the number of papers published in this last decade (Figure 7).

The increase used of these solvents is due to their unique chemical properties such as low melting point, low vapor pressure, chemical and thermal stability, polarity, and miscibility solubility. Moreover, they present a 100% atom economy, low environmental impact due to their low cost and simplicity to produce using natural and environmentally friendly substances [163]. Polarity is a key property to solubilizing compounds in an extraction process. The HBAs and HBDs used to for the DESs play a significant role in the final polarity, as well as the addition of water [112,164]. For example, Craveiro et al showed that DESs composed of choline chloride and organic acids were more polar than the ones with sugars [118]. Depending on the target compound, the DESs can be more hydrophobic or hydrophilic. Most of them are hydrophilic, however the use of hydrophobic DESs in extractions is increasing [165]. Hydrophobic DESs based on choline bromide with different chain lengths were synthesized to extract phytochemicals from Cannabis sativa. The hydrophobic and hydrophilic features of the DESs facilitated the interactions with polar and non-polar compounds present in the plant increasing the extraction efficiency [166].

Searching the application of the DESs for bioactive compounds extraction in 2023, many applications were developed to retrieve bioactive compounds from natural sources with pharmaceutical interest using these solvents (Table 1).

From the components used to synthesize the DESs, choline chloride as the HBA was by far the most used one, however caution must be taken when using this compound since it is forbidden for cosmetic applications [167,168].

Most of the authors found that the extractions with DESs, independently of the extraction method, have high yields when compared with the use of traditional solvents (methanol, ethanol, acetone, etc.) with advantages of being used at milder temperatures without flammable solvents and lower extraction times [169,170]. Liu et al [171] screen twenty-seven DESs combined with UAE to retrieve scutellarin from Erigerontis Herba, an herb used in Chinese medicine. From the DESs screened, twenty-five had a higher extraction yield than the conventional solvents (methanol and 75% ethanol solution). Vinci et al. [172] used DESs to extract antioxidant compounds from dark chocolate. The DES of betaine and choline chloride showed a 35% higher extraction yield than traditional solvents.

Evidently, that are several factors that influence the extraction yield and components extracted. Some of the most influential are the biomass-to-solvent ratio, the time and temperature of the extraction, the type and ratio of HBA and HBD, the viscosity and density of the DES, water content, and pH [170,172,173]. Chagnoleau et al [167] screened fifteen DESs for extracting antioxidants from out-of-calibre kiwifruits. The authors studied the influence of water on the extraction and demonstrated that up to 40% of water could be added to the system without compromising the antioxidant activity of the extracts. The number and type of hydrogen bonds present in the DESs has a profound impact, not only in the extraction yield but also in the nature of bioactive compound to be extracted. Theoretical studies demonstrated that hydrogen and π-bonds were the main factors affecting the extraction of hesperidin from orange peel using a DES of triethanolamine: 4-methoxyphenol (1:1) with a water content of 35% [174].

One of the advantages associated with DESs is their capacity to be tailor-made solvents, i.e., by varying the HBA and HBD different bioactive compounds can be extracted [175]. This selectivity of the DESs can be explained by different chemical interactions with the target compounds [170]. Ojeda et al. [176] used mango by-product to extract bioactive compounds using DESs. To understand the relation between viscosity and the hydrogen bonds in the DESs they did a theoretical study and found out that the hydrogen bonds have a determining effect on the viscosity of the viscosity of the DES and in the specificity of the compounds extracted. The selectivity of the DESs in extraction was showed by Santos-Martín et al. [177] that used two DESs, one composed by lactate, sodium acetate and water, and a second composed by choline chloride and oxalic acid, to extract phenolic compounds from blueberry leaves using UAE. The authors conclude that not only the DESs had superior performance for the recovery of phenolic compounds when compared to traditional organic solvents, but also, that the lactic-based DES enabled the extraction of a wide range of hydroxycinnamic acids and flavonol derivatives, whereas the choline-based DES was selective towards anthocyanins.

The higher extraction yields and selectivity that the extractions with DESs present can be explained by the damage that the DES provokes in the cell wall of the vegetable material. Scanning electron microscopy (SEM) was used to analyze the surface of the material before and after the extraction with DESs and compared with the extraction performed with conventional organic solvents, the materials extracted with DESs showed more pores and cracks due to the penetration of the DES into the cell structure due to the high hydrogen bonding, van der Waals forces and ionic interactions [178,179]. Chen et al. [180] studied the mechanism of extraction of artemisinin from the leaves of Artemisia annua L. by SEM and ¹H nuclear magnetic resonance and verify that the hydrogen reformation and the pant tissue destruction play vital roles during the extraction process. The penetration of the DESs in the cell wall due to strong interactions is also possible when hydrophobic DESs are used. In this case the van der Waals interactions are the main mechanism [181]. The extraction performance is enhanced when the DESs are used in conjunction with UAE. In these cases, there is a synergetic effect between the cavitation phenomenon of the ultrasound and the ability of the DESs to bond to the target molecules [171,182,183].

The extracts with DESs also presented better results in terms of antimicrobial activity [170,179,184], antiproliferative activity in tumour Caco-2 cells and normal human keratinocyte cells [185], lower toxicity and increase bioavailability of the target compounds when administered to rats [186], higher antioxidant, [184] and anti-inflammatory effects as well as inhibitory effects against α-glucosidase and pancreatic lipase [187] when compared to the extracts from the traditional solvents. The enhanced biological activity of the DESs extracts can be explained by the higher content of bioactive compounds [187,188]. Duarte et al [189] studied the effect of extracts rich in polyphenols from a UAE based DES extraction and concluded those extracts showed a large antioxidant activity and a significant effect on the growth of Gram-positive and Gram-negative bacteria. The biological activity of the DESs extracts opens the possibility of using these extracts in pharmaceutical industry, to prevent and treat diseases or to develop new drugs.

Another important advantage of the extracts containing DESs is their stability. Some studies were performed to study this parameter and found that the DESs extracts have a longer shelf-life when compared with conventional solvents extracts [133,185]. This stabilization was due to the ability of the DESs to stabilize the phenolic compounds [190]. Lee et al [165] used leaves from kale to extract several health-promoting phytochemicals using natural DESs with different hydrophilicity/hydrophobicity. The DES extracts provided the greatest stability of the bioactive polyphenols by retaining 91.7% and 88.6% of the original content after 30 days of storage at 4ºC and 25ºC, respectively.

One of the main drawbacks in using the DESs in an industrial scale is the difficulty to remove the DESs from the extracts and to recycle them form further use, however, new methods are being developed to be able to separate the DESs from the extracts and to reused them for subsequent extractions. Anstiss et al. [133] screen twenty-two hydrophobic DESs to extract fatty acids and instead of removing the DES from the extracts, reused the entire extract 5 times without losing the extraction efficiency. Another approach was used by Lanjekar et al. [191] that used a macroporous resin to entrap the compound of interest, glycyrrhizin acid and separate it from the extracts. They reused the DESs in two cycles with more than 90% of the extraction efficiency. Water as antisolvent was used to recover and recycle DES based-on choline chloride used to extract rutin form from Saphora japonica L. with a yield of 94.9%. The DESs was reused at least three times without loss of extraction yield [192]. The anti-solvent method was also used by Liu et al. [171] to recover 71.7% of scutellarin after the DES extraction. Biphasic systems were also developed to separate the DESs from the extracts. These systems were comprised of an aqueous DES phase, and a second organic phase (e.g., ethyl acetate). Using this system, the target compounds are extracted to the aqueous phase and then transferred to the hydrophobic phase. In this way the target compounds are separated from the DES and at the same time the extraction yield is increased [193]. Other approaches were developed to separate the DESs from the extracts based on temperature [194], pH [195], and CO₂ [174]. Tang et al [194] prepared hydrophobic temperature-switchable DESs to extract Lycium barbarum polysaccharides. After the extraction, the temperature was changed to separate the two phases, the aqueous, rich in polysaccharides and the hydrophobic one with the DESs. The authors reused the DESs five extraction cycles with a percentage of recovery after the fifth cycle of 80.2%. Ca et al. [195] used pH responsive polymeric DESs with a phosphate salt to extract aromatic amino acids. By adjusting the pH, the DESs was separated and reused for further extractions. Wang et al [174] synthesized CO₂-responsive DESs to extract hesperidin from orange peel. The reversible phase transformation of the DES solution was achieved by bubbling CO₂/N₂ in the DES solution to recover the hesperidin from the top phase.

The type of extraction method used is the most significant factor affecting the extraction outcome. Methods such as MAE and UAE are often used in conjunction with the DESs. These methods drastically reduced the solvent requirement and extraction time, thereby minimizing energy consumption and cost compared with traditional extraction processes [196]. Additionally, the cavitation phenomenon of the UAE in junction with the DESs proven to be highly effective on disrupting the cell wall, increasing the extraction efficiency [178].

MCAE using DESs was used to extract bioactive compounds from tea leaves, results showed that the extraction was complete within 20 seconds, therefore this method as the potential to be a powerful tool for efficient extraction of thermal sensitive bioactive compounds [197].

The choice of the DESs to use in the extraction process is normally based on trial-and-error approaches or acquired knowledge. However, some theoretical approaches have been used to choose the DESs. These approaches assume that the best DES to extract a pre-determine compound is one that allow to have a higher number of interactions (hydrogen bonds, van der Waals/electrostatic forces, etc.) with the target compound. Fan et al. [180] used COSMO-RS (short for COnductor like Screening MOdel for Real Solvents) to design DESs capable of extract artemisinin from the leaves of Artemisia annua L. The program was used to predict the thermophysical properties and extraction performance of the DESs by simulating the intermolecular forces of the target in the mixed system. COSMO-RS was also used to screen twenty-two hydrophobic DESs by predicting their interactions with the two omega-3 polyunsaturated fatty acids [133]. In both cases, the DESs screen showed to be efficient and selective towards the target compound.

Table 1. Application of DESs as extraction solvents of bioactive compounds from natural sources in the last year.

Best DES(s) (molar ratio)	Natural source	Target compound	Extraction technique(s)	Reference
ChCl:PG (1:2)	Wolfberry	Rhamnogalacturonan-I (RG-I) pectin	CE	[179]
LA:water	Vine shoots	Phytochemicals (proanthocyanins, stilbenes, hydroxycinnamic acids, and flavonols)	UAE/CE	[189]
ChChl:MA (1:1) ChChl:GLY (1:2)	Orange	Bioactive compounds and ascorbic acid	CE	[190]
LA:GLY (1:2)	Evodia lepta	Alkaloids	MAE	[193]
ChChl:EG (1:3)	Abalone viscera	Polysaccharides	UAE	[198]
TET: LAU (1:1)	Lycium barbarum	Polysaccharides	UAE	[194]
Terpenoid-based	Rosmarinus officinalis L.	Bioactive oxidants	UAE	[181]
CAM:GLY (1:1)	Micromelum minutum	Polysaccharides	CE	[199]
ChChl:GLY (1:2) ChCl:LAC (1:3) ChCl:CA (1:1).	Apple	Bioactive compounds	UAE	[169]
BetHCl:EG (1:10)	Kiwifruits	Antioxidants	CE	[167]
[N4444]Cl:AA (1:1).	---	Aromatic amino acids	---	[195]
TEA:4-MP (1:1)	Orange	Hesperidin	UAE	[174]
ChCl-ACA (1:2)	Peanut	Flavonoids	UAE	[183]
BET-LAC	Astragalus-Safflower	hydroxysafflor yellow A, anhydrosafflor yellow B, eleutheroside B, calycosin-7-O-glucoside, kaempferol-3-O-rutinoside, ononin, calycosin, astraganoside, carthamin	UAE	[186]
LACT: NAACE:H2O (3:1:2) ChCl:OA (1:1)	Blueberry	Phenolic compounds	UAE	[177]
ChCl:ACE (1:4)	Erigerontis Herb	Scutellarin	UAE	[171]
ChCl:CA (1:1)	Curcuma longa	Curcuminoids	MAE	[196]
BET:GLY (1:3)	Kale	Polyphenols	Solid/Liquid	[165]
BET:MA:PRO (1:1:1)	Propolis	Bioactive compunds	UAE	[200]
ChCl:Gly (1:2) ChCl:URE(1:2)	Rhamnus alaternus	Polyphenols	CE	[184]
ChCl:EG(1:2)	Sophora japonica L.	Rutin	CE	[192]
BENZAC:FEN (1:4)	Artemisia annua L.	Artemisinin	CE	[192]
β-ALA:MA:H2O (1:1:3)	Mango	Phenolic compounds	CE/ UAE	[176]
CA:MA:H2O (1:1:10)	Persimmon	Fibers/Antioxidants	UAE	[201]
ChCl:GLY (1:1)	Avocado	Phenolics/Carbohydrates	MAE	[202]
ChCl:GLY (1:2)	Kiwifruits	Polyphenols	UAE	[187]
ChCl:GLY (1:2)	Spice	Polyphenols	UAE	[203]
ChCl:ACA (1:4)	Tarragon	Bioactive compounds	CE	[204]
LAC:GLY (1:2)	Foxtail millet Husk	Bioactive compounds	UAE	[178]
ChCl:GLY (1:1)	Violet Potato	Bioactive compounds	UAE/MAE	[185]
BET:TEG (1:2) ChCl:PROP (1:2)	Coffee ground	Polyphenols	CE	[170]
BET: FRU (1:1)	Dark Chocolate	Bioactive compounds	UAE	[172]
ACA:GLU (2:1) ACA:GLY (2:!)	Tea	Tannins/Flavonoids/Terpenoids	UAE/EAE	[188]
MEN:LID (1:1)	Perna canaliculus	Omega-3	CE	[133]
DDBAC:LA (1:3)	Gardenia	Bioactive Compounds	CE	[205]
[N1 1 16 (2OH)+][Br−]:THY (1:2)	Cannabis sativa L.	Phytochemicals	Microextraction	[166]
ChCl:BUT (1:4)	C. vulgaris	Bioactive compounds	CE	[173]
ChCl:MA (1:1) ChCl:LAC (1:3)	Aralia elata	Triterpene Saponins	CE	[206]
CA:GLY:H2O (1:4:10/15/20)	Chamaenerion angustifolium (L.) Scop.	Bioactive compounds	UAE	[207]
CA:MAL (1:2)	Nettle	Bioactive Compounds	UAE	[208]
ChCl:LAC (1:1)	Iris sibirica L.	Bioactive compounds	CE	[209]
ChCl:LAC (1:2)	Edible Feijoa	Flavonoids	CE	[210]
ChCl:LAC (1:4)	Mexican Oregano	Flavonoids	UAE	[211]
ChCl-PHE (1:3)	Hop	Polyphenols	CE/UAE/UHE	[212]
ChCl:GLU (1:0.8)	Capsicum chinense	Polyphenols	UAE	[175]
ChCl:LAC (1:1)	Glycyrrhiza glabra	Glycyrrhizic acid	MAE	[175]
ChCl:MA (1:2) ChCl:CA (1:2) ChCl:4BUT (1:2)	Fenugreek	Flavonoids	UAE	[213]
ChCl:LAC (1:2)	black mulberry	Flavonoids/Phenolics	UAE	[182]
ChCl:4BUT (1:2)	Tea	Flavonoids/Alkaloids/Catechins	MCAE	[197]

Table 1. Application of DESs as extraction solvents of bioactive compounds from natural sources in the last year.

Best DES(s) (molar ratio)	Natural source	Target compound	Extraction technique(s)	Reference
ChCl:PG (1:2)	Wolfberry	Rhamnogalacturonan-I (RG-I) pectin	CE	[179]
LA:water	Vine shoots	Phytochemicals (proanthocyanins, stilbenes, hydroxycinnamic acids, and flavonols)	UAE/CE	[189]
ChChl:MA (1:1) ChChl:GLY (1:2)	Orange	Bioactive compounds and ascorbic acid	CE	[190]
LA:GLY (1:2)	Evodia lepta	Alkaloids	MAE	[193]
ChChl:EG (1:3)	Abalone viscera	Polysaccharides	UAE	[198]
TET: LAU (1:1)	Lycium barbarum	Polysaccharides	UAE	[194]
Terpenoid-based	Rosmarinus officinalis L.	Bioactive oxidants	UAE	[181]
CAM:GLY (1:1)	Micromelum minutum	Polysaccharides	CE	[199]
ChChl:GLY (1:2) ChCl:LAC (1:3) ChCl:CA (1:1).	Apple	Bioactive compounds	UAE	[169]
BetHCl:EG (1:10)	Kiwifruits	Antioxidants	CE	[167]
[N4444]Cl:AA (1:1).	---	Aromatic amino acids	---	[195]
TEA:4-MP (1:1)	Orange	Hesperidin	UAE	[174]
ChCl-ACA (1:2)	Peanut	Flavonoids	UAE	[183]
BET-LAC	Astragalus-Safflower	hydroxysafflor yellow A, anhydrosafflor yellow B, eleutheroside B, calycosin-7-O-glucoside, kaempferol-3-O-rutinoside, ononin, calycosin, astraganoside, carthamin	UAE	[186]
LACT: NAACE:H2O (3:1:2) ChCl:OA (1:1)	Blueberry	Phenolic compounds	UAE	[177]
ChCl:ACE (1:4)	Erigerontis Herb	Scutellarin	UAE	[171]
ChCl:CA (1:1)	Curcuma longa	Curcuminoids	MAE	[196]
BET:GLY (1:3)	Kale	Polyphenols	Solid/Liquid	[165]
BET:MA:PRO (1:1:1)	Propolis	Bioactive compunds	UAE	[200]
ChCl:Gly (1:2) ChCl:URE(1:2)	Rhamnus alaternus	Polyphenols	CE	[184]
ChCl:EG(1:2)	Sophora japonica L.	Rutin	CE	[192]
BENZAC:FEN (1:4)	Artemisia annua L.	Artemisinin	CE	[192]
β-ALA:MA:H2O (1:1:3)	Mango	Phenolic compounds	CE/ UAE	[176]
CA:MA:H2O (1:1:10)	Persimmon	Fibers/Antioxidants	UAE	[201]
ChCl:GLY (1:1)	Avocado	Phenolics/Carbohydrates	MAE	[202]
ChCl:GLY (1:2)	Kiwifruits	Polyphenols	UAE	[187]
ChCl:GLY (1:2)	Spice	Polyphenols	UAE	[203]
ChCl:ACA (1:4)	Tarragon	Bioactive compounds	CE	[204]
LAC:GLY (1:2)	Foxtail millet Husk	Bioactive compounds	UAE	[178]
ChCl:GLY (1:1)	Violet Potato	Bioactive compounds	UAE/MAE	[185]
BET:TEG (1:2) ChCl:PROP (1:2)	Coffee ground	Polyphenols	CE	[170]
BET: FRU (1:1)	Dark Chocolate	Bioactive compounds	UAE	[172]
ACA:GLU (2:1) ACA:GLY (2:!)	Tea	Tannins/Flavonoids/Terpenoids	UAE/EAE	[188]
MEN:LID (1:1)	Perna canaliculus	Omega-3	CE	[133]
DDBAC:LA (1:3)	Gardenia	Bioactive Compounds	CE	[205]
[N1 1 16 (2OH)+][Br−]:THY (1:2)	Cannabis sativa L.	Phytochemicals	Microextraction	[166]
ChCl:BUT (1:4)	C. vulgaris	Bioactive compounds	CE	[173]
ChCl:MA (1:1) ChCl:LAC (1:3)	Aralia elata	Triterpene Saponins	CE	[206]
CA:GLY:H2O (1:4:10/15/20)	Chamaenerion angustifolium (L.) Scop.	Bioactive compounds	UAE	[207]
CA:MAL (1:2)	Nettle	Bioactive Compounds	UAE	[208]
ChCl:LAC (1:1)	Iris sibirica L.	Bioactive compounds	CE	[209]
ChCl:LAC (1:2)	Edible Feijoa	Flavonoids	CE	[210]
ChCl:LAC (1:4)	Mexican Oregano	Flavonoids	UAE	[211]
ChCl-PHE (1:3)	Hop	Polyphenols	CE/UAE/UHE	[212]
ChCl:GLU (1:0.8)	Capsicum chinense	Polyphenols	UAE	[175]
ChCl:LAC (1:1)	Glycyrrhiza glabra	Glycyrrhizic acid	MAE	[175]
ChCl:MA (1:2) ChCl:CA (1:2) ChCl:4BUT (1:2)	Fenugreek	Flavonoids	UAE	[213]
ChCl:LAC (1:2)	black mulberry	Flavonoids/Phenolics	UAE	[182]
ChCl:4BUT (1:2)	Tea	Flavonoids/Alkaloids/Catechins	MCAE	[197]