Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Ingestion of Bean Leaves Reduces Metabolic Complications and Restores Intestinal Integrity in C57BL/6 Mice with Obesity Induced by a High-Fat, High-Fructose Diet

Perla Viridiana Ocampo-Anguiano
ORCID logo ,
Laura Lizeth Victoria-Ruiz
,
Rosalía Reynoso-Camacho
,
Andrea Margarita Olvera-Ramírez
ORCID logo ,
Nuria Elizabeth Rocha-Guzmán
,
Minerva Ramos-Gómez
* ORCID logo ,
Santiaga Marisela Ahumada-Solórzano
* ORCID logo
Version 1 : Received: 16 December 2023 / Approved: 18 December 2023 / Online: 18 December 2023 (14:03:43 CET)

A peer-reviewed article of this Preprint also exists.

Ocampo-Anguiano, P.V.; Victoria-Ruiz, L.L.; Reynoso-Camacho, R.; Olvera-Ramírez, A.M.; Rocha-Guzmán, N.E.; Ramos-Gómez, M.; Ahumada-Solórzano, S.M. Ingestion of Bean Leaves Reduces Metabolic Complications and Restores Intestinal Integrity in C57BL/6 Mice with Obesity Induced by a High-Fat and High-Fructose Diet. Nutrients 2024, 16, 367. Ocampo-Anguiano, P.V.; Victoria-Ruiz, L.L.; Reynoso-Camacho, R.; Olvera-Ramírez, A.M.; Rocha-Guzmán, N.E.; Ramos-Gómez, M.; Ahumada-Solórzano, S.M. Ingestion of Bean Leaves Reduces Metabolic Complications and Restores Intestinal Integrity in C57BL/6 Mice with Obesity Induced by a High-Fat and High-Fructose Diet. Nutrients 2024, 16, 367.

Abstract

The consumption of foods with fiber and phenolic compounds can be part of treating obesity through interaction with the gastrointestinal tract. Treatment with common bean leaves (BL) for obesity was evaluated in mice on a high-fat, high-fructose diet (HFFD). The proximal and phytochemical characterization of BL was determined. Obesity was induced with a HFFD for 14 weeks. Immediately after, the animals were supplemented with BL (10%) or standard diet (SD) as a strategy to seem a healthy diet (12 additional weeks). Changes in body composi-tion, lipid profile, and intestinal integrity were analyzed. BL contained 27.2% dietary fiber, total phenolics (475.04 mg/100 g), and saponins (2.2 mg/100 g). The VAT consumption of the BL group decreased by 52% compared to the HFFD group. Additionally, triglyceride concentrations were 23% lower in the BL consumption group compared to the HFFD group. The improvement in lipid profile was attributed to the (1.77-fold) higher fecal lipid excretion in the BL consumption group compared to the HFFD group. Likewise, pancreatic lipase decreased by 29%. Furthermore, BL supplementation reduced the level of IL-6 (4.4-fold) and FITC-dextran in serum by 50% compared with that in the HFFD group. Metabolic endotoxemia was inhibited after BL supplementation (-33%) compared to the HFFD group. BL consumption as a treatment in obese mice reduces adipose tissue accumulation and improves the lipid profile. Furthermore, we report for the first time that BL consumption improves intestinal integrity.

Keywords

bean leaves; obesity treatment; intestinal integrity

Subject

Biology and Life Sciences, Food Science and Technology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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