Version 1
: Received: 12 December 2023 / Approved: 13 December 2023 / Online: 13 December 2023 (08:08:50 CET)
How to cite:
Zubova, M.Y.; Goncharuk, E.A.; Nechaeva, T.L.; Aksenova, M.A.; Katanskaya, V.M.; Kazantseva, V.V.; Zagoskina, N.V. The Influence of Light Intensity and Duration on the Formation of Primary and Secondary Metabolites in Tea Plant Cell Culture. Preprints2023, 2023120958. https://doi.org/10.20944/preprints202312.0958.v1
Zubova, M.Y.; Goncharuk, E.A.; Nechaeva, T.L.; Aksenova, M.A.; Katanskaya, V.M.; Kazantseva, V.V.; Zagoskina, N.V. The Influence of Light Intensity and Duration on the Formation of Primary and Secondary Metabolites in Tea Plant Cell Culture. Preprints 2023, 2023120958. https://doi.org/10.20944/preprints202312.0958.v1
Zubova, M.Y.; Goncharuk, E.A.; Nechaeva, T.L.; Aksenova, M.A.; Katanskaya, V.M.; Kazantseva, V.V.; Zagoskina, N.V. The Influence of Light Intensity and Duration on the Formation of Primary and Secondary Metabolites in Tea Plant Cell Culture. Preprints2023, 2023120958. https://doi.org/10.20944/preprints202312.0958.v1
APA Style
Zubova, M.Y., Goncharuk, E.A., Nechaeva, T.L., Aksenova, M.A., Katanskaya, V.M., Kazantseva, V.V., & Zagoskina, N.V. (2023). The Influence of Light Intensity and Duration on the Formation of Primary and Secondary Metabolites in Tea Plant Cell Culture. Preprints. https://doi.org/10.20944/preprints202312.0958.v1
Chicago/Turabian Style
Zubova, M.Y., Varvara V. Kazantseva and Natalia V. Zagoskina. 2023 "The Influence of Light Intensity and Duration on the Formation of Primary and Secondary Metabolites in Tea Plant Cell Culture" Preprints. https://doi.org/10.20944/preprints202312.0958.v1
Abstract
Tea plants (Camellia sinensis L.) are economically important horticultural crops for industrial use. The drink (tea) obtained from them is used all over the world, and its functional activity is determined by the polyphenols content and composition. Its accumulation is regulated by the action of many environmental factors. This paper presents data on the different intensity (50, 75, and 100 µmol·m−2·s−1) and duration of light exposure on tea plant in vitro cultures. Changes in morphophysiological characteristics, accumulation of carbohydrates and polyphenols, including their monomeric (phenylpropanoids, flavanols) and oligomeric (proanthocyanidins) forms, were studied. The malondialdehyde and photosynthetic pigments (chlorophyll a and b) contents were also analyzed. In the calli grown in light conditions, chloroplasts and anthocyanoplasts formation was noted, which was especially pronounced under the highest illumination intensity. A significant similarity between the growth of callus cultures, the carbohydrates and polyphenols accumulation at 50 and 75 µmol·m−2·s−1 was established. Which wasn’t characteristic of the calli grown at 100 µmol·m−2·s−1. The stress reaction absence in tea callus cultures has been demonstrated, which may be a consequence of the phenolic antioxidants’ accumulation in them. The obtained data evidence that their potential ability to accumulate primary (carbohydrates) and secondary (polyphenols) metabolites depends on the light exposure intensity and duration.
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.