Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Comparative Genomic Analysis of Colletotrichum lini Strains with Different Virulence on Flax

Ekaterina M. Dvorianinova
* ORCID logo ,
Elizaveta A. Sigova
ORCID logo ,
Timur D. Mollaev
,
Tatiana A. Rozhmina
,
Ludmila P. Kudryavtseva
,
Roman O. Novakovskiy
ORCID logo ,
Anastasia A. Turba
ORCID logo ,
Daiana A. Zhernova
,
Elena V. Borkhert
,
Elena N. Pushkova
,
Nataliya V. Melnikova
ORCID logo ,
Alexey A. Dmitriev
* ORCID logo
Version 1 : Received: 12 December 2023 / Approved: 13 December 2023 / Online: 13 December 2023 (05:07:10 CET)

A peer-reviewed article of this Preprint also exists.

Dvorianinova, E.M.; Sigova, E.A.; Mollaev, T.D.; Rozhmina, T.A.; Kudryavtseva, L.P.; Novakovskiy, R.O.; Turba, A.A.; Zhernova, D.A.; Borkhert, E.V.; Pushkova, E.N.; Melnikova, N.V.; Dmitriev, A.A. Comparative Genomic Analysis of Colletotrichum lini Strains with Different Virulence on Flax. J. Fungi 2024, 10, 32. Dvorianinova, E.M.; Sigova, E.A.; Mollaev, T.D.; Rozhmina, T.A.; Kudryavtseva, L.P.; Novakovskiy, R.O.; Turba, A.A.; Zhernova, D.A.; Borkhert, E.V.; Pushkova, E.N.; Melnikova, N.V.; Dmitriev, A.A. Comparative Genomic Analysis of Colletotrichum lini Strains with Different Virulence on Flax. J. Fungi 2024, 10, 32.

Abstract

Colletotrichum lini is a flax fungal pathogen, comprising differently virulent strains. In this work, we sequenced the genomes of three C. lini strains of different virulence: high virulent #390-1, medium virulent #757, and low virulent #771. We obtained more than 100× genome coverage with Oxford Nanopore Technologies reads and more than 50× genome coverage with Illumina data. To construct high-quality genomes, several assembly strategies were tested. The final assemblies were obtained using the Canu – Racon ×2 – Medaka – Polca scheme and were 54.0-55.3 Mb in length, contained 26-32 contigs, had an N50 > 5 Mb and the BUSCO completeness > 96%. Comparative analysis of the constructed genomes showed high similarity between mitochondrial and nuclear genomes. However, a rearrangement event and the loss of a 0.9 Mb contig were revealed. The candidate effectors were predicted in sets of proteins received after genome annotation. The obtained genome sequences of C. lini strains extend the knowledge on the genetic diversity of Colletotrichum species and are the basis for establishing molecular mechanisms of pathogenicity of the flax pathogen and effective control of flax anthracnose.

Keywords

Colletotrichum lini; anthracnose; flax pathogen; virulence; Nanopore sequencing; de novo genome assembly

Subject

Biology and Life Sciences, Ecology, Evolution, Behavior and Systematics

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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