Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Sclerostin Alters Tumor Cell Characteristics of Oral Squamous Cell Carcinoma and May Be a Key Player in Local Bone Invasion

Uwe Schirmer
ORCID logo ,
Sina Allegra Schneider
,
Tatjana Khromov
ORCID logo ,
Felix Bremmer
,
Boris Schminke
,
Henning Schliephake
ORCID logo ,
Klaus Liefeith
,
Phillipp Brockmeyer
* ORCID logo
Version 1 : Received: 11 December 2023 / Approved: 12 December 2023 / Online: 12 December 2023 (13:12:40 CET)

A peer-reviewed article of this Preprint also exists.

Schirmer, U.; Schneider, S.A.; Khromov, T.; Bremmer, F.; Schminke, B.; Schliephake, H.; Liefeith, K.; Brockmeyer, P. Sclerostin Alters Tumor Cell Characteristics of Oral Squamous Cell Carcinoma and May Be a Key Player in Local Bone Invasion. Cells 2024, 13, 137. Schirmer, U.; Schneider, S.A.; Khromov, T.; Bremmer, F.; Schminke, B.; Schliephake, H.; Liefeith, K.; Brockmeyer, P. Sclerostin Alters Tumor Cell Characteristics of Oral Squamous Cell Carcinoma and May Be a Key Player in Local Bone Invasion. Cells 2024, 13, 137.

Abstract

Localized jawbone invasion is a milestone in the progression of oral squamous cell carcinoma (OSCC). The factors that promote this process are not well understood. Sclerostin is known to be involved in bone metabolism and there are preliminary reports of its involvement in bone tumors and metastasis. To identify a possible involvement of sclerostin in the bone invasion process of OSCC, sclerostin expression was analyzed in vitro in two different human OSCC tumor cell lines by qRT-PCR, and the effect of recombinant human (rh)-sclerostin treatment on tumor cell capabil-ities was evaluated using proliferation, migration, and invasion assays. Undifferentiated human mesenchymal stem cells (hMSCs) were osteogenically differentiated and co-cultured with OSCC tumor cells to demonstrate potential interactions on migration characteristics. Sclerostin expres-sion was evaluated in clinical cases by immunohistochemistry at the OSCC jawbone interface in a cohort of 15 patients. Sclerostin expression was detected in both OSCC tumor cell lines in vitro and was also detected at the OSCC jawbone interface in clinical cases. Tumor cell proliferation rate, migration and invasion ability were increased by rh-sclerostin treatment. The migration rate of tumor cells co-cultured with osteogenically differentiated hMSCs was increased. The re-sults presented are the first data suggesting a possible involvement of sclerostin in the bone inva-sion process of OSCC, which needs to be further investigated

Keywords

oral squamous cell carcinoma; bone invasion; sclerostin; SOST

Subject

Biology and Life Sciences, Life Sciences

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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