Braik, A.; Serna-Duque, J.A.; Nefzi, A.; Aroui, S.; Esteban, M.Á. Potential Therapeutic Use of Dermaseptin S4 from the Frog Phyllomedusa Sauvagii and Its Derivatives against Bacterial Pathogens in Fish. Journal of Applied Microbiology 2024, doi:10.1093/jambio/lxae222.
Braik, A.; Serna-Duque, J.A.; Nefzi, A.; Aroui, S.; Esteban, M.Á. Potential Therapeutic Use of Dermaseptin S4 from the Frog Phyllomedusa Sauvagii and Its Derivatives against Bacterial Pathogens in Fish. Journal of Applied Microbiology 2024, doi:10.1093/jambio/lxae222.
Braik, A.; Serna-Duque, J.A.; Nefzi, A.; Aroui, S.; Esteban, M.Á. Potential Therapeutic Use of Dermaseptin S4 from the Frog Phyllomedusa Sauvagii and Its Derivatives against Bacterial Pathogens in Fish. Journal of Applied Microbiology 2024, doi:10.1093/jambio/lxae222.
Braik, A.; Serna-Duque, J.A.; Nefzi, A.; Aroui, S.; Esteban, M.Á. Potential Therapeutic Use of Dermaseptin S4 from the Frog Phyllomedusa Sauvagii and Its Derivatives against Bacterial Pathogens in Fish. Journal of Applied Microbiology 2024, doi:10.1093/jambio/lxae222.
Abstract
One of the main families of antimicrobial peptides (AMPs) derived from the skin secretions of Hylidae frogs are dermaseptins. Among them, dermaseptin S4 (DS4) is characterized by its broad spectrum of activity against bacteria, protozoa and fungi. This study determined the physicochemical properties of the native peptide DS4(1-28) and its derivatives [DS4(1-28)a and DS4(1-26)a] isolated from the skin of the frog Phyllomedusa sauvagii and examined their antimicrobial properties against two marine pathogenic bacteria (Vibrio harveyi and V. anguillarum). The results indicate that DS4(1-26)a has high bacteriicdal activity against the tested strains and low hemolytic activity (> 30 % lysis at the highest tested concentration of 100 µg mL-1) compared to the other two peptides. In addition, all three peptides affected the membrane and cell wall integrity of both pathogenic bacteria, causing leakage of cellular contents, with DS4(1-26)a having the most severe impact. These properties were corroborated by tansmission electron microscopy and by the variation of cations in their binding sites due to the effect of AMPs. These results suggest that DS4 and its derivatives, particularly the truncated and amidated peptide DS4(1-26)a, could potentially be effective in the treatment of infections caused by these marine pathogenic bacteria. Future experiments are required to validate the use of DS4 in vivo in the prevention of fish bacterial diseases.
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