Lactoferricin B Combined with Antibiotics Exhibits Leukemic Selectivity and Antimicrobial Activity

Version 1 : Received: 8 December 2023 / Approved: 9 December 2023 / Online: 11 December 2023 (06:06:21 CET)
Version 2 : Received: 27 January 2024 / Approved: 29 January 2024 / Online: 29 January 2024 (08:40:14 CET)

A peer-reviewed article of this Preprint also exists.

Lica, J.J.; Gucwa, K.; Heldt, M.; Stupak, A.; Maciejewska, N.; Ptaszyńska, N.; Łęgowska, A.; Pradhan, B.; Gitlin-Domagalska, A.; Dębowski, D.; Jakóbkiewicz-Banecka, J.; Rolka, K. Lactoferricin B Combined with Antibiotics Exhibits Leukemic Selectivity and Antimicrobial Activity. Molecules 2024, 29, 678. Lica, J.J.; Gucwa, K.; Heldt, M.; Stupak, A.; Maciejewska, N.; Ptaszyńska, N.; Łęgowska, A.; Pradhan, B.; Gitlin-Domagalska, A.; Dębowski, D.; Jakóbkiewicz-Banecka, J.; Rolka, K. Lactoferricin B Combined with Antibiotics Exhibits Leukemic Selectivity and Antimicrobial Activity. Molecules 2024, 29, 678.

Abstract

The fusion of penetrating peptides (PPs), e.g., cell penetration peptides (CPPs), or antimicrobial peptides (AMPs) together with antimicrobial agents is an expanding research field. Specific AMPs, such as lactoferricin B (LfcinB), have demonstrated strong antibacterial, antifungal, and antiparasitic activity, as well as valuable anticancer activity, proving beneficial in the development of anticancer conjugates. The resulting conjugates offer potential dual functionality, acting as both an anticancer and an antimicrobial agent. This is especially necessary in cancer treatment where microbial infections pose a critical risk. Leukemic cells frequently exhibit altered outer lipid membranes compared to healthy cells, making them more sensitive to compounds that interfere with their membrane. In this communication, we revisited and reanalyzed our earlier research on LfcinB and its conjugates. Furthermore, we carried out new experiments with a specific focus on cell proliferation, changes in membrane asymmetric phosphatidylserine location, intracellular reactive oxygen species (ROS) generation, mitochondrial functions, and in vitro bacterial topoisomerase inhibition.

Keywords

alternative leukemia treatment; antileukemic agents; antimicrobial peptide; dual-activity compounds; bioconjugates; cell penetration peptide; ciprofloxacin; hematopoietic stem cell transplantation; levofloxacin; lactoferricin B; fluconazole; fluoroquinolones.

Subject

Medicine and Pharmacology, Hematology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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