DNA Barcoding as a Plant Identification Method

Stavroula Letsiou; Panagiotis Madesis; Efstathios Vasdekis; Cinzia Montemurro; Maria E. Grigoriou; George Skavdis; Vasillios Moussis; Antonios E. Koutelidakis; Andreas G. Tzakos

doi:10.20944/preprints202312.0536.v1

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preprints.org > biology and life sciences > life sciences > doi: 10.20944/preprints202312.0536.v1

Preprint Review Version 1 Preserved in Portico This version is not peer-reviewed

DNA Barcoding as a Plant Identification Method

Antonios E. Koutelidakis

Andreas G. Tzakos

Version 1 : Received: 7 December 2023 / Approved: 7 December 2023 / Online: 8 December 2023 (03:34:41 CET)

A peer-reviewed article of this Preprint also exists.

Letsiou, S.; Madesis, P.; Vasdekis, E.; Montemurro, C.; Grigoriou, M.E.; Skavdis, G.; Moussis, V.; Koutelidakis, A.E.; Tzakos, A.G. DNA Barcoding as a Plant Identification Method. Appl. Sci. 2024, 14, 1415. Letsiou, S.; Madesis, P.; Vasdekis, E.; Montemurro, C.; Grigoriou, M.E.; Skavdis, G.; Moussis, V.; Koutelidakis, A.E.; Tzakos, A.G. DNA Barcoding as a Plant Identification Method. Appl. Sci. 2024, 14, 1415.

Abstract

In the last two decades, plant taxonomy has bloomed, following the development of a novel technique, namely DNA Barcoding. DNA barcodes are standardized sequences, ideally unique, coding or non-coding, either from the genome of the organism or from its organelles, that are used to identify/classify an organismal group; in short the method includes amplification of the DNA barcode, sequencing and comparison with a reference database containing the relevant sequences from different species. In plants, the use a universal DNA barcode, such as COI which is used in animals, has not been achieved so far. Several DNA barcodes from single loci as well as combinations of barcodes have been successfully used in various settings; additionally, as next generation sequencing becomes affordable, very long sequences- super barcodes, such as the chloroplast genome are being used successfully especially to distinguish between close species. In this review we summarize the work done on DNA barcoding in plants and discuss different approaches used so far.

Keywords

DNA Barcoding; species identification; taxonomy; plants; molecular markers

Subject

Biology and Life Sciences, Life Sciences

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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