Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Unveiling the Probiotic Potential of Streptococcus thermophilus MCC0200: Insights from In-vitro Studies corroborated with Genome Analysis

Neelam Kapse
ORCID logo ,
Vaidehi Pisu
,
Tanisha Dhakephalkar
,
Prajakta Margale
,
Deepa Shetty
,
Shilpa Wagh
,
Sumit Dagar
,
Prashant Dhakephalkar
*
Version 1 : Received: 7 December 2023 / Approved: 7 December 2023 / Online: 7 December 2023 (13:58:01 CET)

A peer-reviewed article of this Preprint also exists.

Kapse, N.; Pisu, V.; Dhakephalkar, T.; Margale, P.; Shetty, D.; Wagh, S.; Dagar, S.; Dhakephalkar, P.K. Unveiling the Probiotic Potential of Streptococcus thermophilus MCC0200: Insights from In Vitro Studies Corroborated with Genome Analysis. Microorganisms 2024, 12, 347. Kapse, N.; Pisu, V.; Dhakephalkar, T.; Margale, P.; Shetty, D.; Wagh, S.; Dagar, S.; Dhakephalkar, P.K. Unveiling the Probiotic Potential of Streptococcus thermophilus MCC0200: Insights from In Vitro Studies Corroborated with Genome Analysis. Microorganisms 2024, 12, 347.

Abstract

Streptococcus thermophilus is widely used as a starter culture in the dairy industry and has garnered attention as a beneficial bacterium owing to its health-promoting functionalities in humans. In this study, the probiotic potential of S. thermophilus MCC0200, isolated from a dairy product, was investigated through a combinatorial approach of in-vitro and in-silico studies. MCC0200 demonstrated the ability to survive harsh gastro-intestinal transit (GIT), adhere to intestinal mucosa and exert health-promoting traits in in-vitro studies. These findings were corroborated with in-silico evidence, wherein MCC0200 genome harboured genes associated with tolerance to GIT conditions, intestinal adhesion and colonization. Genome mapping also highlighted the ability of MCC0200 to produce compounds advantageous for host (folate, bacteriocins), to release enzymes that can reinforce the host ability (antioxidative enzymes), to metabolize food components that can be harmful to sensitive people (lactose). MCC0200 also demonstrated a positive effect on reducing cholesterol levels, proving to be a potential candidate for food and pharmaceutical applications. Absence of transmissible antibiotic resistance genes and virulence genes underscored the GRAS nature of MCC0200. This study explored the potential of Streptococcus thermophilus for its probable applications as a probiotic beyond the dairy industry.

Keywords

Streptococcus thermophilus; gastrointestinal transit; adhesion; health-promoting; Anti-hypercholesterolemic activity; GRAS; genome

Subject

Biology and Life Sciences, Life Sciences

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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