Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Three-Dimensional Cultures for Collecting CNS and PNS Glial Secreted Factors, Vesicles and Scaffolds Free of Animal Serum Components

Cinzia Cocola
* ,
Tasnim Mohamed
,
Eleonora Piscitelli
,
Paride Pelucchi
,
Edoardo Abeni
,
Ettore Mosca
ORCID logo ,
Guiseppina De Petro
ORCID logo ,
Giovanni Porta
,
James Kehler
,
Martin Götte
ORCID logo ,
Ileana Zucchi
,
Valerio Magnaghi
ORCID logo ,
Rolland Reinbold
*
Version 1 : Received: 22 November 2023 / Approved: 4 December 2023 / Online: 4 December 2023 (04:14:33 CET)

How to cite: Cocola, C.; Mohamed, T.; Piscitelli, E.; Pelucchi, P.; Abeni, E.; Mosca, E.; Petro, G.D.; Porta, G.; Kehler, J.; Götte, M.; Zucchi, I.; Magnaghi, V.; Reinbold, R. Three-Dimensional Cultures for Collecting CNS and PNS Glial Secreted Factors, Vesicles and Scaffolds Free of Animal Serum Components. Preprints 2023, 2023120115. https://doi.org/10.20944/preprints202312.0115.v1 Cocola, C.; Mohamed, T.; Piscitelli, E.; Pelucchi, P.; Abeni, E.; Mosca, E.; Petro, G.D.; Porta, G.; Kehler, J.; Götte, M.; Zucchi, I.; Magnaghi, V.; Reinbold, R. Three-Dimensional Cultures for Collecting CNS and PNS Glial Secreted Factors, Vesicles and Scaffolds Free of Animal Serum Components. Preprints 2023, 2023120115. https://doi.org/10.20944/preprints202312.0115.v1

Abstract

Glial cells provide physical and chemical support and protection for neurons. Secreted neurotrophic factors, scaffolds and vesicles regulate in addition to normal homeostasis, repair following neural tissue injury or degeneration. We previously demonstrated that human CNS glia have revascularization capacity by inducing endothelial cell sprouting and microchanneling driven by glial cell secreted components. The clinical potential of using secreted factors and vesicles generated by glial cells in conditioned media was shown by their re-normalization of pathological blood vessels and 3D tissue wound healing and remodeling capacity. To identify secreted components of glial cells and how CNS and PNS glial cells differentially regulate neural tissue homeostasis, disease and injury response, we developed a novel culture method for various types of glial cells that allow for isolating and characterizing secreted glial components, free of animal factors and contaminants normally associated with serum-based culture conditions. Our culture method will enable to identify secreted components using ultrasensitive RNA, glycoconjugate and protein analysis technologies. The characterization of glial secreted factors involved microchanneling may help in development of new clinical therapies for glial and neural cells or axons to migrate and reform connections.

Keywords

glial cells; neurogenesis; vasculogenic mimicry; microchanneling; central nervous system; peripheral nervous system; 2D and 3D serum replacement culture; glycoconjugates; vesicles

Subject

Biology and Life Sciences, Biology and Biotechnology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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