PreprintBrief ReportVersion 1Preserved in Portico This version is not peer-reviewed
Allele Frequencies and the Probability of Identity for 18 STR Loci Generated via the Canine GenotypesTM Panel 2.1 Kit in Golden Retriever, Miniature Dachshund, and Shiba Inu Groups for Forensic Applications
Version 1
: Received: 1 December 2023 / Approved: 1 December 2023 / Online: 1 December 2023 (13:37:35 CET)
How to cite:
Kunita, F.; Udagawa, C.; Inagaki, T.; Suzuki, H.; Bonkobara, M.; Omi, T. Allele Frequencies and the Probability of Identity for 18 STR Loci Generated via the Canine GenotypesTM Panel 2.1 Kit in Golden Retriever, Miniature Dachshund, and Shiba Inu Groups for Forensic Applications. Preprints2023, 2023120089. https://doi.org/10.20944/preprints202312.0089.v1
Kunita, F.; Udagawa, C.; Inagaki, T.; Suzuki, H.; Bonkobara, M.; Omi, T. Allele Frequencies and the Probability of Identity for 18 STR Loci Generated via the Canine GenotypesTM Panel 2.1 Kit in Golden Retriever, Miniature Dachshund, and Shiba Inu Groups for Forensic Applications. Preprints 2023, 2023120089. https://doi.org/10.20944/preprints202312.0089.v1
Kunita, F.; Udagawa, C.; Inagaki, T.; Suzuki, H.; Bonkobara, M.; Omi, T. Allele Frequencies and the Probability of Identity for 18 STR Loci Generated via the Canine GenotypesTM Panel 2.1 Kit in Golden Retriever, Miniature Dachshund, and Shiba Inu Groups for Forensic Applications. Preprints2023, 2023120089. https://doi.org/10.20944/preprints202312.0089.v1
APA Style
Kunita, F., Udagawa, C., Inagaki, T., Suzuki, H., Bonkobara, M., & Omi, T. (2023). Allele Frequencies and the Probability of Identity for 18 STR Loci Generated via the Canine GenotypesTM Panel 2.1 Kit in Golden Retriever, Miniature Dachshund, and Shiba Inu Groups for Forensic Applications. Preprints. https://doi.org/10.20944/preprints202312.0089.v1
Chicago/Turabian Style
Kunita, F., Makoto Bonkobara and Toshinori Omi. 2023 "Allele Frequencies and the Probability of Identity for 18 STR Loci Generated via the Canine GenotypesTM Panel 2.1 Kit in Golden Retriever, Miniature Dachshund, and Shiba Inu Groups for Forensic Applications" Preprints. https://doi.org/10.20944/preprints202312.0089.v1
Abstract
Similarly to Europe and the United States, the need for the forensic DNA identification of dogs is increasing in Japan. Because few studies use commercial genotyping kits, we examine the effectiveness of individual DNA identification using the Canine Genotypes Panel 2.1 Kit on limited samples of dogs bred in Japan. We used Genomic DNA extracted from blood in non-related 50 Golden Retrievers, 50 Miniature Dachshunds, and 50 Shiba Inus bred in Japan. We investigated 18 canine STR markers (PEZ02, PEZ17, FH2017, FH2309, PEZ05, FH2001, FH2328, FH2004, FH2361, PEZ21, FH2054, FH3377, FH2107, FH2088, vWF.X, FH2010, PEZ16, and FH3313) and one sex-related marker (ZFX.Y) according to the manufacturer’s instructions. Allele frequency, He, Ho, p-value, PD, PE, PIC, and MP were calculated for each marker. Random Match probability based on 18 STR loci was subsequently estimated to be 3.257 × 10−16 in Golden Retrievers, 3.933 × 10−18 in Miniature Dachshunds, and 2.107 × 10−18 in the Shiba Inus breed. There are a few studies that have used this kit in Japan. The results suggest that the kit with 18 autosomal STR loci and one sex marker is effective in forensic applications.
Keywords
dog DNA identification; STR; golden retriever; miniature dachshunds; shiba inus
Subject
Biology and Life Sciences, Animal Science, Veterinary Science and Zoology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
