Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Sa-β-Gal in Kidney Tubules as a Predictor of Renal Outcome in Patients with Chronic Kidney Disease

Pasquale Esposito
ORCID logo ,
Daniela Picciotto
,
Daniela Verzola
,
Giacomo Garibotto
* ORCID logo ,
Emanuele Luigi Parodi
,
Antonella Sofia
,
Francesca Costigliolo
,
Gabriele Gaggero
,
Valentina Zanetti
,
Michela Saio
,
Francesca Viazzi
ORCID logo
Version 1 : Received: 30 November 2023 / Approved: 1 December 2023 / Online: 1 December 2023 (05:32:39 CET)

A peer-reviewed article of this Preprint also exists.

Esposito, P.; Picciotto, D.; Verzola, D.; Garibotto, G.; Parodi, E.L.; Sofia, A.; Costigliolo, F.; Gaggero, G.; Zanetti, V.; Saio, M.; Viazzi, F. SA-β-Gal in Kidney Tubules as a Predictor of Renal Outcome in Patients with Chronic Kidney Disease. J. Clin. Med. 2024, 13, 322. Esposito, P.; Picciotto, D.; Verzola, D.; Garibotto, G.; Parodi, E.L.; Sofia, A.; Costigliolo, F.; Gaggero, G.; Zanetti, V.; Saio, M.; Viazzi, F. SA-β-Gal in Kidney Tubules as a Predictor of Renal Outcome in Patients with Chronic Kidney Disease. J. Clin. Med. 2024, 13, 322.

Abstract

.Cellular senescence has emerged as an important driver of aging and age-related disease in the kidney. The activity of β-galactosidase at pH 6 (SA-β-Gal) is a classic maker of senescence in cellular biology. However the predictive role of kidney tissue SA-β-Gal on eGFR loss in chronic kidney disease (CKD) is still not understood. We retrospectively studied the expression of SA-β-Gal in kidney biopsies obtained in a cohort [n=29] of incident patients who were followed up for 3 years as for standard of care. SA-β-Gal staining was approximately 4-fold higher in the tubular compartment of patients with CKD vs. controls [26.0 ± 9 vs. 7.4 ± 6% positive tubuli in patients vs. controls; p < 0.025]. Tubular expressions of SA-β-Gal, but not proteinuria at the time of biopsy correlated with eGFR loss at follow-up; moreover, SA-β-Gal expression in more than 30% of kidney tubules was associated with fast progressive kidney disease. In summary, our study shows that SA-β-Gal is upregulated in the kidney tubular compartment of adult patients affected by CKD, and suggests that tubular SA-β-Gal is associated with accelerated loss of renal function.

Keywords

senescence; cell; chronic kidney disease; kidney tubules

Subject

Medicine and Pharmacology, Urology and Nephrology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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