Yu Isoda, Mika K. Kaneko, Tomohiro Tanaka, Hiroyuki Suzuki, and Yukinari Kato. Epitope Mapping of an Anti-ferret Podoplanin Monoclonal Antibody Using the PA Tag-Substituted Analysis. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy.Dec 2023.189-193.http://doi.org/10.1089/mab.2023.0026
Yu Isoda, Mika K. Kaneko, Tomohiro Tanaka, Hiroyuki Suzuki, and Yukinari Kato. Epitope Mapping of an Anti-ferret Podoplanin Monoclonal Antibody Using the PA Tag-Substituted Analysis. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy.Dec 2023.189-193.http://doi.org/10.1089/mab.2023.0026
Yu Isoda, Mika K. Kaneko, Tomohiro Tanaka, Hiroyuki Suzuki, and Yukinari Kato. Epitope Mapping of an Anti-ferret Podoplanin Monoclonal Antibody Using the PA Tag-Substituted Analysis. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy.Dec 2023.189-193.http://doi.org/10.1089/mab.2023.0026
Yu Isoda, Mika K. Kaneko, Tomohiro Tanaka, Hiroyuki Suzuki, and Yukinari Kato. Epitope Mapping of an Anti-ferret Podoplanin Monoclonal Antibody Using the PA Tag-Substituted Analysis. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy.Dec 2023.189-193.http://doi.org/10.1089/mab.2023.0026
Abstract
In small animal models of severe acute respiratory syndrome coronaviruses (SARS-CoV and SARS-CoV-2) infection, ferrets (Mustela putorius furo) have been used to investigate the pathogenesis. Podoplanin (PDPN) is an essential marker in lung type I alveolar epithelial cells, kidney podocytes, and lymphatic endothelial cells. Monoclonal antibodies (mAbs) against ferret PDPN (ferPDPN) are useful for the pathological analyses of those tissues. We previously established an anti-ferPDPN mAb, PMab-292 using the Cell-Based Immunization and Screening (CBIS) method. In this study, we determined the critical epitope of PMab-292 using flow cytometry. The N-terminal ferPDPN deletion mutants analysis revealed that the Val34 is located at the N-terminus of the PMab-292 epitope. Furthermore, the PA tag-substituted analysis (PA scanning) showed that Asp39 is located at the C-terminus of PMab-292 epitope. The epitope sequence (34-VRPEDD-39) also exists between Val26 and Asp31 of ferPDPN, indicating that PMab-292recognizes the tandem repeat of the VRPEDD sequence of ferPDPN.
Keywords
ferret podoplanin; monoclonal antibody; epitope; PA scanning
Subject
Medicine and Pharmacology, Other
Copyright:
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