Effect of Silver Nanoparticles and Essential Oils against Multidrug Resistant Staphylococcus pseudintermedius: An In Vitro Study

Gabriele Meroni; Giulia Laterza; Alexios Tsikopoulos; Konstantinos Tsikopoulos; Sara Vitalini; Barbara Scaglia; Marcello Iriti; Luigi Bonizzi; Piera Anna Martino; Alessio Soggiu

doi:10.20944/preprints202311.1528.v1

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preprints.org > biology and life sciences > immunology and microbiology > doi: 10.20944/preprints202311.1528.v1

Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Effect of Silver Nanoparticles and Essential Oils against Multidrug Resistant Staphylococcus pseudintermedius: An In Vitro Study

Gabriele Meroni

^* ,

Giulia Laterza

Alexios Tsikopoulos

Konstantinos Tsikopoulos

Version 1 : Received: 22 November 2023 / Approved: 23 November 2023 / Online: 23 November 2023 (11:37:45 CET)

A peer-reviewed article of this Preprint also exists.

Meroni, G.; Laterza, G.; Tsikopoulos, A.; Tsikopoulos, K.; Vitalini, S.; Scaglia, B.; Iriti, M.; Bonizzi, L.; Martino, P.A.; Soggiu, A. Antibacterial Potential of Essential Oils and Silver Nanoparticles against Multidrug-Resistant Staphylococcus pseudintermedius Isolates. Pathogens 2024, 13, 156. Meroni, G.; Laterza, G.; Tsikopoulos, A.; Tsikopoulos, K.; Vitalini, S.; Scaglia, B.; Iriti, M.; Bonizzi, L.; Martino, P.A.; Soggiu, A. Antibacterial Potential of Essential Oils and Silver Nanoparticles against Multidrug-Resistant Staphylococcus pseudintermedius Isolates. Pathogens 2024, 13, 156.

Abstract

Staphylococcus pseudintermedius (SP) is an emergent zoonotic agent associated with multidrug resistance (MDR). This work aimed to describe the antibacterial activity of four essential oils (EOs) and silver nanoparticles (AgNPs) against SP. Fifteen SP strains were tested. The four EOs, namely Rosmarinus officinalis (RO), Juniperus communis (GI), Citrus sinensis (AR) and Abies alba (AB), and AgNPs were used alone and in combination to determine the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) against SP. All strains presented MDR and methicillin resistance. Among the antibiotic cohort, only rifampicin, doxycycline and amikacin were effective. When EOs were tested alone, AB showed the lowest MIC followed by GI, RO and AR (0.23%±0.3; 0.5%±0.51; 0.79%±0.39; 0.93%±0.63). MBC was increased with the following order: AB, GI, AR and RO (3.56%±4.87; 4.93%±6.27; 8.53%±7.2; 10.34%±5.31). MIC and MBC values for AgNPs were 10.74 mg/L±4.23; 261.05 mg/L±172.74. In combination, the MIC and MBC values were lower for all compounds. In conclusion, EOs and AgNPs could be considered as promising antibacterial alternatives to antibiotics.

Keywords

MRSP; MDR; essential oil; silver nanoparticles

Subject

Biology and Life Sciences, Immunology and Microbiology

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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