Preprint Article Version 1 Preserved in Portico This version is not peer-reviewed

Genetic Diversity, Pathogenicity and Host Resistance of Fusarium Species from Soybean in Canada

Longfei Wu
ORCID logo ,
Sheau-Fang Hwang
,
Stephen E. Strelkov
ORCID logo ,
Rudolph Fredua-Agyeman
ORCID logo ,
Sanghoen Oh
,
Richard R. Bélanger
,
Owen Wally
ORCID logo ,
Yong Min Kim
* ORCID logo
Version 1 : Received: 22 November 2023 / Approved: 23 November 2023 / Online: 23 November 2023 (11:06:49 CET)

How to cite: Wu, L.; Hwang, S.; Strelkov, S.E.; Fredua-Agyeman, R.; Oh, S.; Bélanger, R.R.; Wally, O.; Kim, Y.M. Genetic Diversity, Pathogenicity and Host Resistance of Fusarium Species from Soybean in Canada. Preprints 2023, 2023111514. https://doi.org/10.20944/preprints202311.1514.v1 Wu, L.; Hwang, S.; Strelkov, S.E.; Fredua-Agyeman, R.; Oh, S.; Bélanger, R.R.; Wally, O.; Kim, Y.M. Genetic Diversity, Pathogenicity and Host Resistance of Fusarium Species from Soybean in Canada. Preprints 2023, 2023111514. https://doi.org/10.20944/preprints202311.1514.v1

Abstract

Fusarium spp. are commonly associated with the root rot complex of soybean (Glycine max). Previous surveys identified six common Fusarium species from Manitoba, including F. oxysporum, F. redolens, F. graminearum, F. solani, F. avenaceum and F. acuminatum. This study aimed to determine their pathogenicity, assess host resistance and evaluate the genetic diversity of Fusarium spp. isolated from Canada. Pathogenicity was tested on two cultivars, ‘Akras’ (moderate resistance) and ‘B150Y1’ (susceptible), under greenhouse conditions. The virulence of the fungal isolates varied, with root rot severities ranging from 1.5 to 3.3 on a 0-4 scale. The six species were used to screen a panel of 20 Canadian soybean cultivars for resistance in a greenhouse. Cluster and principal component analyses were conducted based on the same traits as for the pathogenicity study. Two of the cultivars, ‘P15T46R2’ and ‘B150Y1’, were consistently found to be tolerant to F. oxysporum, F. redolens, F. graminearum and F. solani. To investigate the incidence and prevalence of Fusarium spp. in Canada, fungi were isolated from 106 soybean fields surveyed across Manitoba, Saskatchewan, Ontario and Quebec. Two-hundred twenty-one Fusarium isolates were identified, with phylogenetic analyses indicating diversity of the isolates in the major soybean production regions of Canada.

Keywords

Fusarium spp.; genetic diversity; pathogenicity; phylogenetic analyses; resistance; root rot; Sanger sequencing; soybean

Subject

Biology and Life Sciences, Plant Sciences

Copyright: This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Download PDF Download Supplementary

Comments (0)

We encourage comments and feedback from a broad range of readers. See criteria for comments and our Diversity statement.

Leave a public comment
Send a private comment to the author(s)
* All users must log in before leaving a comment
Views 0
Downloads 0
Comments 0
Metrics 0
Get PDF Supplementary Files Cite Share 0
Bookmark BibSonomy Mendeley Reddit Delicious
×
Alerts
Notify me about updates to this article or when a peer-reviewed version is published.
We use cookies on our website to ensure you get the best experience.
Read more about our cookies here.