Version 1
: Received: 22 November 2023 / Approved: 23 November 2023 / Online: 23 November 2023 (10:51:36 CET)
How to cite:
Steinbach, A.; József, K.; Urban, P.; Palkovics, T.; György, S. Molecular Responses of The Eukaryotic Cell Line INT407 on the Internalised C. jejuni - the other Side of the Coin. Preprints2023, 2023111510. https://doi.org/10.20944/preprints202311.1510.v1
Steinbach, A.; József, K.; Urban, P.; Palkovics, T.; György, S. Molecular Responses of The Eukaryotic Cell Line INT407 on the Internalised C. jejuni - the other Side of the Coin. Preprints 2023, 2023111510. https://doi.org/10.20944/preprints202311.1510.v1
Steinbach, A.; József, K.; Urban, P.; Palkovics, T.; György, S. Molecular Responses of The Eukaryotic Cell Line INT407 on the Internalised C. jejuni - the other Side of the Coin. Preprints2023, 2023111510. https://doi.org/10.20944/preprints202311.1510.v1
APA Style
Steinbach, A., József, K., Urban, P., Palkovics, T., & György, S. (2023). Molecular Responses of The Eukaryotic Cell Line INT407 on the Internalised <em>C. jejuni</em> - the other Side of the Coin. Preprints. https://doi.org/10.20944/preprints202311.1510.v1
Chicago/Turabian Style
Steinbach, A., Tamás Palkovics and Schneider György. 2023 "Molecular Responses of The Eukaryotic Cell Line INT407 on the Internalised <em>C. jejuni</em> - the other Side of the Coin" Preprints. https://doi.org/10.20944/preprints202311.1510.v1
Abstract
Campylobacter jejuni is a zoonotic bacterium, causing intestinal infection in humans. One characteristic feature of the pathogenicity process is the ability of this bacterium invading the epithelial cells. Several factors have been identified to contribute to this process and some recent analyses revealed changes in the bacterium cell on molecular level. However, our knowledge is still limited about responses of the host that accompany the bacterial internalisation and survival process. This study focused on molecular events on transcriptomic level detected between the 1st and the 3rd hour of internalisation in INT407 epithelial cells. From the 41.769 human genes tested, altogether 19,060 genes and 22,734 pseudogenes and introns were shown by whole transcriptome analysis to be affected in different extent. Most affected functions regulate transcription (28%), signal transduction (21%), apoptosis (15%), immune responses (9%), transmembrane transport (6%), cell-cell signaling (3%), cell-cell adhesions (3%) and carbohydrate metabolism (3%) and to a lesser extent other functions. Our results provide insight into a scenario where, the invaded cell focuses on survival, but, at the same time, flashing the possibility of pushing the process toward the death of the invaded and metabolically disrupted cell.
Keywords
Campylobacter jejuni; INT407 cell line infection; WTA
Subject
Biology and Life Sciences, Immunology and Microbiology
Copyright:
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
